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dc.contributor.authorInstitoris, Etel
dc.contributor.authorFox, Brian W
dc.date.accessioned2011-09-23T17:46:42Z
dc.date.available2011-09-23T17:46:42Z
dc.date.issued1978-07
dc.identifier.citationThe interaction of dianhydrogalactitol with DNA in cultured Yoshida sarcoma cells. 1978, 22 (1):99-107 Chem. Biol. Interact.en
dc.identifier.issn0009-2797
dc.identifier.pmid567539
dc.identifier.doi10.1016/0009-2797(78)90153-9
dc.identifier.urihttp://hdl.handle.net/10541/143016
dc.description.abstractUsing alkaline sucrose gradient sedimentation centrifugation it was found that treatment of Yoshida sarcoma cells in culture for 1 h with increasing concentrations of dianhydrogalactitol (DAG) enhanced the sedimentation rate of DNA in a dose-dependent manner. There was no difference between the amount of protein which co-sedimented with DNA released from treated and untreated cells. When DNA was extracted from the cells using a p-amino-salicylate-phenol mixture, the protein content of DNA seemed not to be affected by DAG. The possibility that DAG could form interstrand cross-linking in cellular DNA was suggested from renaturation studies. The appearance of a fast sedimenting DNA in the alkaline sucrose gradient and the evidence for a cross-linked DNA detected by renaturation technique, only appeared later than 6 h after treatment. A similar delayed effect on the depression in the rate of DNA synthesis was also observed. These data suggest that the inhibition of DNA synthesis may be related to the delayed formation of DNA interstrand cross-linked.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshCells, Cultured
dc.subject.meshCentrifugation, Density Gradient
dc.subject.meshDNA, Neoplasm
dc.subject.meshDianhydrogalactitol
dc.subject.meshNeoplasm Proteins
dc.subject.meshSarcoma, Yoshida
dc.subject.meshSugar Alcohols
dc.subject.meshTime Factors
dc.titleThe interaction of dianhydrogalactitol with DNA in cultured Yoshida sarcoma cells.en
dc.typeArticleen
dc.contributor.departmentPaterson Laboratories, Christie Hospital and Holt Radium Institute, Manchesteren
dc.identifier.journalChemico-Biological Interactionsen
html.description.abstractUsing alkaline sucrose gradient sedimentation centrifugation it was found that treatment of Yoshida sarcoma cells in culture for 1 h with increasing concentrations of dianhydrogalactitol (DAG) enhanced the sedimentation rate of DNA in a dose-dependent manner. There was no difference between the amount of protein which co-sedimented with DNA released from treated and untreated cells. When DNA was extracted from the cells using a p-amino-salicylate-phenol mixture, the protein content of DNA seemed not to be affected by DAG. The possibility that DAG could form interstrand cross-linking in cellular DNA was suggested from renaturation studies. The appearance of a fast sedimenting DNA in the alkaline sucrose gradient and the evidence for a cross-linked DNA detected by renaturation technique, only appeared later than 6 h after treatment. A similar delayed effect on the depression in the rate of DNA synthesis was also observed. These data suggest that the inhibition of DNA synthesis may be related to the delayed formation of DNA interstrand cross-linked.


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