Fractionation of chromatin by differential solubility in dilute salt.
AffiliationPaterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester
MetadataShow full item record
AbstractChromatin prepared from the livers of rats was fractionated on the basis of solubility in dilute NaCl. Neither of the fractions obtained was enriched in newly synthesized DNA. The salt-soluble fraction had a higher protein content (usually up to 50%) relative to the DNA, and contained 72% or more of the rapidly synthesized RNA. This RNA was found to be complexed with the salt-soluble deoxyribonucleoprotein, not merely co-solubilized with it. Also, polylysine-binding studies showed that about 70% or more of the nucleic acid phosphates were accessible as compared to about 40% in the unfractionated chromatin. These properties suggested that the soluble fraction was enriched in activity transcribed chromatin. In contrast molecular hybridization studies showed that the complexity of the DNA and its homology with cDNA transcribed from rat-liver polysomal mRNA were the same as those of DNA from unfractionated chromatin, or from the salt-insoluble fraction. This suggests that the criteria commonly accepted as distinguishing between euchromatin and heterochromatin in vitro are not invariably valid.
CitationFractionation of chromatin by differential solubility in dilute salt. 1978, 5 (3):739-50 Nucleic Acids Res.
JournalNucleic Acids Research
- Further studies on a compact form of salt-soluble deoxyribonucleoprotein.
- Authors: Krueger RC, Allison DP
- Issue date: 1973 Jun 23
- The distribution of nuclear proteins and transcriptionally-active sequences in rat liver chromatin fractions.
- Authors: Mironov NM, Lobanenkov VV, Goodwin GH
- Issue date: 1986 Dec
- Discrimination of several classes of nonhistone proteins in chromatin fractions from liver and thymus nuclei of rats.
- Authors: Weihe A, Schmidt G, von Mickwitz CU, Lindigkeit R
- Issue date: 1982
- [Contractile proteins in the fractions of soluble and insoluble chromatin of liver and thymus].
- Authors: Priiatkina TN, Zarembskaia OR, Ivanova EM, Stepanov MG, Panteleeva NS
- Issue date: 1983 Nov
- Polylysine titration of rat liver chromatin fractions after DNase II digestion.
- Authors: Vauhkonen M, Hemminki K
- Issue date: 1977 Apr