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dc.contributor.authorNikaido, O
dc.contributor.authorFox, Brian W
dc.date.accessioned2011-08-23T11:56:39Z
dc.date.available2011-08-23T11:56:39Z
dc.date.issued1976-07
dc.identifier.citationThe time course of DNA repair following methyl nitro-nitrosoguanidine (MNNG) treatment of p388f lymphoma cells in culture. II. DNA repair synthesis and its correlation with strand breakage. 1976, 14 (1-2):47-55 Chem Biol Interacten
dc.identifier.issn0009-2797
dc.identifier.pmid954140
dc.identifier.doi10.1016/0009-2797(76)90023-5
dc.identifier.urihttp://hdl.handle.net/10541/140473
dc.description.abstractRepair synthesis has been followed in P388F cell DNA following treatment with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) using 5-iodo-deoxyuridine (IUdR) and [3H] thymidine combination. A pattern of repair synthesis was obtained, similar in its timing to the initial and medial stages of single-strand break formation and rejoining within the first 24 h after treatment. A higher base insertion frequency in relation to single-strand breaks occurs during the medial stage of repair. However, a fundamental problem with the use of iododeoxyuridine and [3H] thymidine ([3H] TdR) as a suitable measure of repair synthesis was encountered and a potential source of error in such experiments was observed.
dc.language.isoenen
dc.subject.meshCell Line
dc.subject.meshDNA Repair
dc.subject.meshDNA Replication
dc.subject.meshDNA, Single-Stranded
dc.subject.meshIdoxuridine
dc.subject.meshKinetics
dc.subject.meshMethylnitronitrosoguanidine
dc.subject.meshNitrosoguanidines
dc.subject.meshThymidine
dc.titleThe time course of DNA repair following methyl nitro-nitrosoguanidine (MNNG) treatment of p388f lymphoma cells in culture. II. DNA repair synthesis and its correlation with strand breakage.en
dc.typeArticleen
dc.identifier.journalChemico-Biological Interactionsen
html.description.abstractRepair synthesis has been followed in P388F cell DNA following treatment with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) using 5-iodo-deoxyuridine (IUdR) and [3H] thymidine combination. A pattern of repair synthesis was obtained, similar in its timing to the initial and medial stages of single-strand break formation and rejoining within the first 24 h after treatment. A higher base insertion frequency in relation to single-strand breaks occurs during the medial stage of repair. However, a fundamental problem with the use of iododeoxyuridine and [3H] thymidine ([3H] TdR) as a suitable measure of repair synthesis was encountered and a potential source of error in such experiments was observed.


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