The time course of DNA repair following methyl nitro-nitrosoguanidine (MNNG) treatment of p388f lymphoma cells in culture. II. DNA repair synthesis and its correlation with strand breakage.
dc.contributor.author | Nikaido, O | |
dc.contributor.author | Fox, Brian W | |
dc.date.accessioned | 2011-08-23T11:56:39Z | |
dc.date.available | 2011-08-23T11:56:39Z | |
dc.date.issued | 1976-07 | |
dc.identifier.citation | The time course of DNA repair following methyl nitro-nitrosoguanidine (MNNG) treatment of p388f lymphoma cells in culture. II. DNA repair synthesis and its correlation with strand breakage. 1976, 14 (1-2):47-55 Chem Biol Interact | en |
dc.identifier.issn | 0009-2797 | |
dc.identifier.pmid | 954140 | |
dc.identifier.doi | 10.1016/0009-2797(76)90023-5 | |
dc.identifier.uri | http://hdl.handle.net/10541/140473 | |
dc.description.abstract | Repair synthesis has been followed in P388F cell DNA following treatment with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) using 5-iodo-deoxyuridine (IUdR) and [3H] thymidine combination. A pattern of repair synthesis was obtained, similar in its timing to the initial and medial stages of single-strand break formation and rejoining within the first 24 h after treatment. A higher base insertion frequency in relation to single-strand breaks occurs during the medial stage of repair. However, a fundamental problem with the use of iododeoxyuridine and [3H] thymidine ([3H] TdR) as a suitable measure of repair synthesis was encountered and a potential source of error in such experiments was observed. | |
dc.language.iso | en | en |
dc.subject.mesh | Cell Line | |
dc.subject.mesh | DNA Repair | |
dc.subject.mesh | DNA Replication | |
dc.subject.mesh | DNA, Single-Stranded | |
dc.subject.mesh | Idoxuridine | |
dc.subject.mesh | Kinetics | |
dc.subject.mesh | Methylnitronitrosoguanidine | |
dc.subject.mesh | Nitrosoguanidines | |
dc.subject.mesh | Thymidine | |
dc.title | The time course of DNA repair following methyl nitro-nitrosoguanidine (MNNG) treatment of p388f lymphoma cells in culture. II. DNA repair synthesis and its correlation with strand breakage. | en |
dc.type | Article | en |
dc.identifier.journal | Chemico-Biological Interactions | en |
html.description.abstract | Repair synthesis has been followed in P388F cell DNA following treatment with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) using 5-iodo-deoxyuridine (IUdR) and [3H] thymidine combination. A pattern of repair synthesis was obtained, similar in its timing to the initial and medial stages of single-strand break formation and rejoining within the first 24 h after treatment. A higher base insertion frequency in relation to single-strand breaks occurs during the medial stage of repair. However, a fundamental problem with the use of iododeoxyuridine and [3H] thymidine ([3H] TdR) as a suitable measure of repair synthesis was encountered and a potential source of error in such experiments was observed. |