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    Human mixed lymphocyte culture using separated lymphocyte populations.

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    Authors
    Potter, M R
    Moore, Michael
    Affiliation
    Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester
    Issue Date
    1977-03
    
    Metadata
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    Abstract
    The ability of human blood lymphocyte populations enriched with T or B cells to act as responder and stimulator populations in the one-way mixed lymphocyte reaction (MLR) was investigated. T- and B-cell-enriched populations were obtained by separation of rosette-forming and non rosette-forming cells and T-cell-enriched populations were also obtained by nylon-fibre column filtration. Using cells prepared by rosette sedimentation, control unseparated and T-cell-enriched populations responded well when stimulated by mitomycin C-treated unseparated cells from a second individual; and stimulation by T- and B-enriched populations generally produced some response, although the magnitude was variable. B-cell-enriched populations gave virtually no response regardless of the composition of the stimulating populations. Nylon-column-enriched T-cell populations responded to stimulation by control unseparated cells but not to T cells purified by the same procedure. T-cell enriched populations prepared by the two methods thus had different activities in the MLR despite containing similar numbers of T cells suggesting that other factors, such as the presence of small numbers of accessory cells, are important in determining the magnitude of the MLR.
    Citation
    Human mixed lymphocyte culture using separated lymphocyte populations. 1977, 32 (3):359-65 Immunology
    Journal
    Immunology
    URI
    http://hdl.handle.net/10541/139101
    PubMed ID
    139361
    Type
    Article
    Language
    en
    ISSN
    0019-2805
    Collections
    All Paterson Institute for Cancer Research

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