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dc.contributor.authorBuchanan, J D
dc.contributor.authorPower, D M
dc.contributor.authorPhillips, G O
dc.contributor.authorDavies, J Vernon
dc.date.accessioned2011-08-01T11:35:06Z
dc.date.available2011-08-01T11:35:06Z
dc.date.issued1978-06
dc.identifier.citationInteraction of aminoacridines with nucleic acids. A pulse-radiolysis study. 1978, 33 (6):551-62 Int. J. Radiat. Biol. Relat. Stud. Phys. Chem. Med.en
dc.identifier.issn0020-7616
dc.identifier.pmid308053
dc.identifier.doi10.1080/09553007814550461
dc.identifier.urihttp://hdl.handle.net/10541/138496
dc.description.abstractPulse radiolysis has been used to study the interaction of aminoacridines with nucleic acids. The data confirm that there are two modes of binding. These are: a weak interaction which has a maximum binding ratio of one site per dye; and a strong binding process effected by both electrostatic and Van der Waals interactions. The limit of this latter, strong binding mode is approximately six sites per dye. The radiation-induced transient absorption spectrum of benzoflavine is characterized by a pronounced bleaching at 440 nm, which is quenched by the addition of nucleic acids. Mechanisms have been proposed for the reactions of both eaq-and .OH with benzoflavine which account both for the observed bleaching of benzoflavine solutions and for the protective effect of nucleic acids. It is proposed that eaq-reacts with benzoflavine to form a stable benzoflavine semiquinone radical and that .OH reacts with subsequent formation of a very stable benzoflavine hydroxycyclohexadienyl radical.
dc.language.isoenen
dc.subject.meshAcridines
dc.subject.meshChemical Phenomena
dc.subject.meshChemistry
dc.subject.meshColoring Agents
dc.subject.meshDNA
dc.subject.meshProflavine
dc.subject.meshPulse Radiolysis
dc.subject.meshRNA
dc.titleInteraction of aminoacridines with nucleic acids. A pulse-radiolysis study.en
dc.typeArticleen
dc.contributor.departmentPaterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester.en
dc.identifier.journalInternational Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicineen
html.description.abstractPulse radiolysis has been used to study the interaction of aminoacridines with nucleic acids. The data confirm that there are two modes of binding. These are: a weak interaction which has a maximum binding ratio of one site per dye; and a strong binding process effected by both electrostatic and Van der Waals interactions. The limit of this latter, strong binding mode is approximately six sites per dye. The radiation-induced transient absorption spectrum of benzoflavine is characterized by a pronounced bleaching at 440 nm, which is quenched by the addition of nucleic acids. Mechanisms have been proposed for the reactions of both eaq-and .OH with benzoflavine which account both for the observed bleaching of benzoflavine solutions and for the protective effect of nucleic acids. It is proposed that eaq-reacts with benzoflavine to form a stable benzoflavine semiquinone radical and that .OH reacts with subsequent formation of a very stable benzoflavine hydroxycyclohexadienyl radical.


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