Interaction of aminoacridines with nucleic acids. A pulse-radiolysis study.
AffiliationPaterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester.
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AbstractPulse radiolysis has been used to study the interaction of aminoacridines with nucleic acids. The data confirm that there are two modes of binding. These are: a weak interaction which has a maximum binding ratio of one site per dye; and a strong binding process effected by both electrostatic and Van der Waals interactions. The limit of this latter, strong binding mode is approximately six sites per dye. The radiation-induced transient absorption spectrum of benzoflavine is characterized by a pronounced bleaching at 440 nm, which is quenched by the addition of nucleic acids. Mechanisms have been proposed for the reactions of both eaq-and .OH with benzoflavine which account both for the observed bleaching of benzoflavine solutions and for the protective effect of nucleic acids. It is proposed that eaq-reacts with benzoflavine to form a stable benzoflavine semiquinone radical and that .OH reacts with subsequent formation of a very stable benzoflavine hydroxycyclohexadienyl radical.
CitationInteraction of aminoacridines with nucleic acids. A pulse-radiolysis study. 1978, 33 (6):551-62 Int. J. Radiat. Biol. Relat. Stud. Phys. Chem. Med.
JournalInternational Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine
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