• Login
    View Item 
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ChristieCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjects

    My Account

    LoginRegister

    Local Links

    The Christie WebsiteChristie Library and Knowledge Service

    Statistics

    Display statistics

    Factors affecting the growth of Chinese hamster cells in HAT selection media.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Fox, Margaret
    Boyle, John M
    Issue Date
    1976-06
    
    Metadata
    Show full item record
    Abstract
    Factors affecting the efficiency of selection of "revertants" of salvage pathway mutants in media containing amethopterin have been examined. Our V79 Chinese hamster cell line was found to require a significantly higher level of thymidine for optimal growth in such media than has been reported for other cell lines. Hypoxanthine (but not glycine) was also required for reversal of amethopterin toxicity, but levels did not differ significantly from those reported elsewhere. Growth in HAT was also dependent on plating density and serum batch. Our modification (VHAT) was compared with published HAT recipies in back selection reconstruction experiments. A sharp fall in EOR (efficiency of recovery) of wild type cells from mixtures with mutants at plating densities greater than 3500 cells/cm2 (10(5) cells/6 cm dish) was observed for VHAT. EOR with other HAT recipes was lower still, and was affected also by the particular mutant used in the mixture. EMS induced "revertants" were isolated from three 8AZr mutants by plating in VHAT. All revertants were however amethopterin resistant, they were also 8AZ resistant and the mobility of residual HGPRT (as measured by polyacrylamide gel electrophoresis) was similar to that of their 8AZr parents i.e. dissimilar from that in wild type. The modal chromosome number of V79 wild type cells was 21. No significant deviation from this mode was detected in any of the mutant lines examined. The data indicate that the recovery of colonies in HAT from 8AZr mutants does not necessarily indicate that a back mutation in the structural gene for HGPRT has occurred. Thus, the frequency of HAT+ colonies cannot be taken as a direct indication of reversion frequencies.
    Citation
    Factors affecting the growth of Chinese hamster cells in HAT selection media. 1976, 35 (3):445-64 Mutat Res
    Journal
    Mutation Research
    URI
    http://hdl.handle.net/10541/136603
    PubMed ID
    934167
    Type
    Article
    Language
    en
    ISSN
    0027-5107
    Collections
    All Paterson Institute for Cancer Research

    entitlement

    Related articles

    • Recovery of V79 cell clones with different phenotypes in aminopterin- or azaserine-containing media used for the selection of HGPRT revertants.
    • Authors: Randazzo R, Bonatti S, Luccarini G, Abbondandolo A
    • Issue date: 1984 May
    • Gene amplification as a mechanism of reversion at the HPRT locus in V79 Chinese hamster cells.
    • Authors: Zownir O, Fuscoe JC, Fenwick R, Morrow J
    • Issue date: 1984 Jun
    • Characteristics of revertants induced by EMS and u.v. light from a 6-thioguanine resistant HGPRT deficient V79 Chinese hamster cell line.
    • Authors: Hodgkiss RJ, Fox M
    • Issue date: 1980 Feb
    • Requirement for cell dispersion prior to selection of induced azaguanine-resistant colonies of Chinese hamster cells.
    • Authors: Myhr BC, Dipalo JA
    • Issue date: 1975 May
    • Reversion in expression of hypoxanthine-guanine phosphoribosyl transferase following cell hybridization.
    • Authors: Bakay B, Nyhan WL, Croce CM, Koprowski H
    • Issue date: 1975 May
    DSpace software (copyright © 2002 - 2021)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.