• Login
    View Item 
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ChristieCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsProfilesView

    My Account

    LoginRegister

    Local Links

    The Christie WebsiteChristie Library and Knowledge Service

    Statistics

    Display statistics

    Studies on liver chromatin RNA from rats treated with alkylating agents.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Itzhaki, Ruth F
    Barker, Malcolm
    Billington, Robert W
    Issue Date
    1976-12-01
    
    Metadata
    Show full item record
    Abstract
    We have examined firstly some properties of rat liver chromatin RNA and nuclear sap RNA and secondly the incorporation of [3H]orotic acid into the RNA in vivo in control rats and in rats treated with the alkylating agents, N,N-dimethylnitrosamine or methyl methane sulphonate. Half or more of the nuclear RNA is associated with the chromatin and consists mainly of two species: one is labelled and probably comprises "nascent" RNA, and the other is unlabelled and of lower molecular weight. Neither species is attributable to cytoplasmic contamination. Studies with added polylysine with RNAase A and with DNAase I suggest that both species are ironically bound to protein and that the labelled species is not associated with the part of the chromatin DNA most readily degraded by DNAase I. After dimethylnitrosamine treatment, the amount of unlabelled RNA remains constant but the amount of labelled RNA increases after a low dose, and decreases after a high dose. After methyl methane sulphonate treatment, no change occurs in either species. These results can be explained by changes in extent of association of the DNA and protein within the chromatin complex.
    Citation
    Studies on liver chromatin RNA from rats treated with alkylating agents. 1976, 454 (2):362-74 Biochim Biophys Acta
    Journal
    Biochimica et Biophysica Acta
    URI
    http://hdl.handle.net/10541/136597
    PubMed ID
    999910
    Type
    Article
    Language
    en
    ISSN
    0006-3002
    Collections
    All Paterson Institute for Cancer Research

    entitlement

    Related articles

    • Inhibition of rat liver RNA polymerases by action of the methylating agents dimethylnitrosamine in vivo and methyl methanesulfonate in vitro.
    • Authors: Herzog J, Farber JL
    • Issue date: 1976 May
    • Methylation of DNAase-digestible DNA and of RNA in chromatin from rats treated with dimethylnitrosamine.
    • Authors: Galbraith AI, Barker M, Itzhaki RF
    • Issue date: 1979 Feb 27
    • Physicochemical alterations in the conformation of rat liver chromatin induced by carcinogens in vivo.
    • Authors: Nicolini C, Ramanathan R, Kendall F, Murphy J, Parodi S, Sarma DS
    • Issue date: 1976 May
    • Effects of dimethylnitrosamine on RNA synthesis and metabolism in mouse liver.
    • Authors: Garyfallides S, Kyrtopoulos SA, Sekeris CE
    • Issue date: 1984 Nov
    • Studies on liver chromatin from rats treated with dimethylnitrosamine.
    • Authors: Cooper HK, Itzhaki RF
    • Issue date: 1975 Oct 15
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.