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dc.contributor.authorMcMillan, S
dc.contributor.authorFox, Margaret
dc.date.accessioned2011-07-22T11:45:49Z
dc.date.available2011-07-22T11:45:49Z
dc.date.issued1979-03
dc.identifier.citationFailure of caffeine to influence induced mutation frequencies and the independence of cell killing and mutation induction in V79 Chinese hamster cells. 1979, 60 (1):91-107 Mutat Resen
dc.identifier.issn0027-5107
dc.identifier.pmid431556
dc.identifier.urihttp://hdl.handle.net/10541/136590
dc.description.abstractUsing V79 Chinese hamster cells and a replating assay, no effect of caffeine post-treatment on spontaneous or UV- or EMS-induced mutation frequencies to 8-azaguanine resistance was demonstrable. However, considerable potentiation of cell killing was observed. Previous reports that caffeine enhances induced mutation frequencies are explained by an artefact in the situ method used; a similar artefact may also explain the cumulative in situ mutation dose-response curves. Furthermore, the relationship between mutation induction and dose has been shown to be qualitatively distinct from that between cell killing and dose. These differences suggest that cell killing and mutation induction are mediated via independent mechanisms and that pre-mutational lesions may be qualitatively distinct from pre-lethal lesions.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshAzaguanine
dc.subject.meshCaffeine
dc.subject.meshCell Survival
dc.subject.meshCells, Cultured
dc.subject.meshCricetinae
dc.subject.meshDNA Repair
dc.subject.meshDose-Response Relationship, Drug
dc.subject.meshDrug Resistance
dc.subject.meshEthyl Methanesulfonate
dc.subject.meshLung
dc.subject.meshMutation
dc.subject.meshUltraviolet Rays
dc.titleFailure of caffeine to influence induced mutation frequencies and the independence of cell killing and mutation induction in V79 Chinese hamster cells.en
dc.typeArticleen
dc.identifier.journalMutation researchen
html.description.abstractUsing V79 Chinese hamster cells and a replating assay, no effect of caffeine post-treatment on spontaneous or UV- or EMS-induced mutation frequencies to 8-azaguanine resistance was demonstrable. However, considerable potentiation of cell killing was observed. Previous reports that caffeine enhances induced mutation frequencies are explained by an artefact in the situ method used; a similar artefact may also explain the cumulative in situ mutation dose-response curves. Furthermore, the relationship between mutation induction and dose has been shown to be qualitatively distinct from that between cell killing and dose. These differences suggest that cell killing and mutation induction are mediated via independent mechanisms and that pre-mutational lesions may be qualitatively distinct from pre-lethal lesions.


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