• Login
    View Item 
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ChristieCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsProfilesView

    My Account

    LoginRegister

    Local Links

    The Christie WebsiteChristie Library and Knowledge Service

    Statistics

    Display statistics

    Erythropoietin-stimulated erythropoiesis in long-term bone marrow culture.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Eliason, J
    Testa, Nydia G
    Dexter, T Michael
    Issue Date
    1979-10-04
    
    Metadata
    Show full item record
    Abstract
    The proliferation of multipotential haematopoietic stem cells (CFU-S) is possible in some long-term bone marrow cultures. Granulocyte and macrophage progenitor (CFU-C) and megakaryocyte precursor cells (CFU-M) are present in these cultures and undergo full development into mature cells. In contrast, while immature erythroid progenitors ('early' BFU-E) are maintained in long-term culture, none of the more differentiated progeny (CFU-E) have been detected, and no morphologically recognisable erythroid cells have been observed. We now describe a modified culture system in which the 'early' BFU-E develop into 'late' BFU-E in response to added erythropoietin. Further maturation of these cells into CFU-E and non-nucleated erythrocytes can be achieved by mechanical agitation of the long-term cultures or by transferring the cells into dishes which do not allow cell attachment to occur.
    Citation
    Erythropoietin-stimulated erythropoiesis in long-term bone marrow culture. 1979, 281 (5730):382-4 Nature
    Journal
    Nature
    URI
    http://hdl.handle.net/10541/136564
    DOI
    10.1038/281382a0
    PubMed ID
    481602
    Type
    Article
    Language
    en
    ISSN
    0028-0836
    ae974a485f413a2113503eed53cd6c53
    10.1038/281382a0
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

    entitlement

    Related articles

    • Modulation of erythropoiesis and myelopoiesis by exogenous erythropoietin in human long-term marrow cultures.
    • Authors: Mayani H, Guilbert LJ, Janowska-Wieczorek A
    • Issue date: 1990 Mar
    • Blockade of the in vitro effects of testosterone and erythropoietin on Cfu-E and Bfu-E proliferation by pretreatment of the donor rats with cyproterone and flutamide.
    • Authors: Malgor LA, Valsecia M, Vergés E, De Markowsky EE
    • Issue date: 1998
    • Optimal erythroid cell production during erythropoietin treatment of mice occurs by exploiting the splenic microenvironment.
    • Authors: Nijhof W, Goris H, Dontje B, Dresz J, Loeffler M
    • Issue date: 1993 Apr
    • Long-term culture of murine erythropoietic progenitor cells in the absence of an adherent layer.
    • Authors: Wendling F, Shreeve MM, McLeod DL, Axelrad AA
    • Issue date: 1983 Oct 13-19
    • Effects of human recombinant erythropoietin on differentiation and distribution of erythroid progenitor cells on murine medullary and splenic erythropoiesis during hypoxia and post-hypoxia.
    • Authors: Mide SM, Huygens P, Bozzini CE, Fernandez Pol JA
    • Issue date: 2001 Mar-Apr
    DSpace software (copyright © 2002 - 2025)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.