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dc.contributor.authorPhillips, Paul
dc.contributor.authorKumar, Patricia
dc.contributor.authorKumar, Shant
dc.contributor.authorWaghe, M
dc.date.accessioned2011-07-22T10:15:12Z
dc.date.available2011-07-22T10:15:12Z
dc.date.issued1979-09
dc.identifier.citationIsolation and characterization of endothelial cells from rat and cow brain white matter. 1979, 129 (Pt 2):261-72 J Anaten
dc.identifier.issn0021-8782
dc.identifier.pmid500486
dc.identifier.urihttp://hdl.handle.net/10541/136555
dc.description.abstractA procedure is described for obtaining pure cultures of endothelial cells from rat and cow brain white matter. The morphology of the tissue-cultured cells was studied by both light and transmission electron microscopy. Weibel-Palade bodies, described in the literature as specific for endothelial cells, appeared in small numbers in our cultured cells. Autoradiographic, scintillation counting, immunological and histochemical studies were performed. The usefulness of pure endothelial cell cultures is discussed.
dc.language.isoenen
dc.subject.meshAlkaline Phosphatase
dc.subject.meshAnimals
dc.subject.meshBrain
dc.subject.meshCattle
dc.subject.meshCell Separation
dc.subject.meshCells, Cultured
dc.subject.meshEndothelium
dc.subject.meshHistocytochemistry
dc.subject.meshMicroscopy, Electron
dc.subject.meshOrganoids
dc.subject.meshRats
dc.subject.meshThymidine
dc.titleIsolation and characterization of endothelial cells from rat and cow brain white matter.en
dc.typeArticleen
dc.contributor.departmentChristie Hospital and Holt Radium Institute, Manchesteren
dc.identifier.journalJournal of Anatomyen
html.description.abstractA procedure is described for obtaining pure cultures of endothelial cells from rat and cow brain white matter. The morphology of the tissue-cultured cells was studied by both light and transmission electron microscopy. Weibel-Palade bodies, described in the literature as specific for endothelial cells, appeared in small numbers in our cultured cells. Autoradiographic, scintillation counting, immunological and histochemical studies were performed. The usefulness of pure endothelial cell cultures is discussed.


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