Persistence of methylated bases in ribonucleic acid of syrian golden hamster liver after administration of dimethylnitrosamine.
Affiliation
Paterson Laboratories, Christie Hospital and Holt Radium Institute, ManchesterIssue Date
1979-03-01
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Show full item recordAbstract
1. Syrian golden hamster liver ribosomal RNA was isolated up to 96 h after administration of [14C]dimethylnitrosamine at 25 mg/kg or 2.5 mg/kg body weight. 2. The chemical alkyation products, 7-methylguanine, 3-methylcytosine, O6-methylguanosine and 1-methyladenosine, were measured after acidic or enzymic hydrolysis of the RNA to bases or mononucleosides followed by ion-exchange chromatography. 3. Between 7 and 96 h, the relative amounts of alkylation products did not change with time even though the absolute amounts fell by approx. 80% and 51% after the high and low doses respectively. 4. The results suggest that base specific excision repair does not exist for RNA alkylation products in this experimental system.Citation
Persistence of methylated bases in ribonucleic acid of syrian golden hamster liver after administration of dimethylnitrosamine. 1979, 177 (3):967-73 Biochem JJournal
The Biochemical JournalPubMed ID
444213Type
ArticleLanguage
enISSN
0264-6021Collections
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