Show simple item record

dc.contributor.authorGalbraith, A
dc.contributor.authorItzhaki, Ruth F
dc.date.accessioned2011-07-20T14:13:45Z
dc.date.available2011-07-20T14:13:45Z
dc.date.issued1979-12
dc.identifier.citationStudies on histones and non-histone proteins from rats treated with dimethylnitrosamine. 1979, 28 (2-3):309-22 Chem Biol Interacten
dc.identifier.issn0009-2797
dc.identifier.pmid95105
dc.identifier.doi10.1016/0009-2797(79)90171-6
dc.identifier.urihttp://hdl.handle.net/10541/136431
dc.description.abstractA study has been made of the histone and non-histone chromosomal proteins of rat liver after treatment in vivo with dimethylnitrosamine (DMN) (2 mg/kg). DMN was found not to affect histone turnover, as measured by 3H-labelled amino-acids incorporation. A decrease was observed in specific activity of the histones with time after injection of [14C]DMN or [14C]-formate and this was attributable to demethylation of both abnormal and normal methylation sites in these proteins. In the case of the non-histone proteins, DMN was found to increase greatly the turnover of those non-histone proteins loosely associated with chromatin DNA and RNA; turnover of those non-histone proteins tightly bound to chromatin DNA and RNA was unaffected. Demethylation of both normal and abnormal methylation sites was found to take place from both non-histone protein fractions. In the case of the loosely bound non-histone proteins a lower rate of demethylation was observed after DMN treatment.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshChromosomal Proteins, Non-Histone
dc.subject.meshDNA
dc.subject.meshDimethylnitrosamine
dc.subject.meshElectrophoresis, Polyacrylamide Gel
dc.subject.meshHistones
dc.subject.meshLiver
dc.subject.meshMale
dc.subject.meshRNA
dc.subject.meshRats
dc.titleStudies on histones and non-histone proteins from rats treated with dimethylnitrosamine.en
dc.typeArticleen
dc.identifier.journalChemico-Biological Interactionsen
html.description.abstractA study has been made of the histone and non-histone chromosomal proteins of rat liver after treatment in vivo with dimethylnitrosamine (DMN) (2 mg/kg). DMN was found not to affect histone turnover, as measured by 3H-labelled amino-acids incorporation. A decrease was observed in specific activity of the histones with time after injection of [14C]DMN or [14C]-formate and this was attributable to demethylation of both abnormal and normal methylation sites in these proteins. In the case of the non-histone proteins, DMN was found to increase greatly the turnover of those non-histone proteins loosely associated with chromatin DNA and RNA; turnover of those non-histone proteins tightly bound to chromatin DNA and RNA was unaffected. Demethylation of both normal and abnormal methylation sites was found to take place from both non-histone protein fractions. In the case of the loosely bound non-histone proteins a lower rate of demethylation was observed after DMN treatment.


Files in this item

This item appears in the following Collection(s)

Show simple item record