Studies on histones and non-histone proteins from rats treated with dimethylnitrosamine.
| dc.contributor.author | Galbraith, A | |
| dc.contributor.author | Itzhaki, Ruth F | |
| dc.date.accessioned | 2011-07-20T14:13:45Z | |
| dc.date.available | 2011-07-20T14:13:45Z | |
| dc.date.issued | 1979-12 | |
| dc.identifier.citation | Studies on histones and non-histone proteins from rats treated with dimethylnitrosamine. 1979, 28 (2-3):309-22 Chem Biol Interact | en |
| dc.identifier.issn | 0009-2797 | |
| dc.identifier.pmid | 95105 | |
| dc.identifier.doi | 10.1016/0009-2797(79)90171-6 | |
| dc.identifier.uri | http://hdl.handle.net/10541/136431 | |
| dc.description.abstract | A study has been made of the histone and non-histone chromosomal proteins of rat liver after treatment in vivo with dimethylnitrosamine (DMN) (2 mg/kg). DMN was found not to affect histone turnover, as measured by 3H-labelled amino-acids incorporation. A decrease was observed in specific activity of the histones with time after injection of [14C]DMN or [14C]-formate and this was attributable to demethylation of both abnormal and normal methylation sites in these proteins. In the case of the non-histone proteins, DMN was found to increase greatly the turnover of those non-histone proteins loosely associated with chromatin DNA and RNA; turnover of those non-histone proteins tightly bound to chromatin DNA and RNA was unaffected. Demethylation of both normal and abnormal methylation sites was found to take place from both non-histone protein fractions. In the case of the loosely bound non-histone proteins a lower rate of demethylation was observed after DMN treatment. | |
| dc.language.iso | en | en |
| dc.subject.mesh | Animals | |
| dc.subject.mesh | Chromosomal Proteins, Non-Histone | |
| dc.subject.mesh | DNA | |
| dc.subject.mesh | Dimethylnitrosamine | |
| dc.subject.mesh | Electrophoresis, Polyacrylamide Gel | |
| dc.subject.mesh | Histones | |
| dc.subject.mesh | Liver | |
| dc.subject.mesh | Male | |
| dc.subject.mesh | RNA | |
| dc.subject.mesh | Rats | |
| dc.title | Studies on histones and non-histone proteins from rats treated with dimethylnitrosamine. | en |
| dc.type | Article | en |
| dc.identifier.journal | Chemico-Biological Interactions | en |
| html.description.abstract | A study has been made of the histone and non-histone chromosomal proteins of rat liver after treatment in vivo with dimethylnitrosamine (DMN) (2 mg/kg). DMN was found not to affect histone turnover, as measured by 3H-labelled amino-acids incorporation. A decrease was observed in specific activity of the histones with time after injection of [14C]DMN or [14C]-formate and this was attributable to demethylation of both abnormal and normal methylation sites in these proteins. In the case of the non-histone proteins, DMN was found to increase greatly the turnover of those non-histone proteins loosely associated with chromatin DNA and RNA; turnover of those non-histone proteins tightly bound to chromatin DNA and RNA was unaffected. Demethylation of both normal and abnormal methylation sites was found to take place from both non-histone protein fractions. In the case of the loosely bound non-histone proteins a lower rate of demethylation was observed after DMN treatment. |