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dc.contributor.authorSchor, Seth L
dc.contributor.authorCourt, J
dc.date.accessioned2011-07-20T14:04:00Z
dc.date.available2011-07-20T14:04:00Z
dc.date.issued1979-08
dc.identifier.citationDifferent mechanisms in the attachment of cells to native and denatured collagen. 1979, 38:267-81 J Cell Scien
dc.identifier.issn0021-9533
dc.identifier.pmid521466
dc.identifier.urihttp://hdl.handle.net/10541/136408
dc.description.abstractThe attachment of cells to collagen has been reported previously to require the presence of serum and the particular serum protein involved in this process, variously known as CIG, CAP or fibronectin, has been isolated. This conclusion that cell attachment to collagen requires serum (or more precisely, fibronectin) is based on experiments measuring the kinetics of cell attachment to films of collagen. We have measured the kinetics of attachment of HeLa and attachment to films of collagen-containing substrata under a variety of experimental conditions and present evidence that the serum-dependent mechanism of cell attachment described by others is actually only the case for films of denatured collagen, while cell attachment to native collagen fibres occurs by a different, serum-independent, mechanism. The possible relevance of these findings to cell-matrix interactions in vivo is discussed.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshCell Adhesion
dc.subject.meshCollagen
dc.subject.meshCytochalasin B
dc.subject.meshFibronectins
dc.subject.meshHela Cells
dc.subject.meshProtein Denaturation
dc.subject.meshRats
dc.subject.meshTrypsin
dc.subject.meshUrea
dc.titleDifferent mechanisms in the attachment of cells to native and denatured collagen.en
dc.typeArticleen
dc.contributor.departmentPaterson Laboratories, Christie Hospital and Holt Radium Institute, Manchesteren
dc.identifier.journalJournal of cell scienceen
html.description.abstractThe attachment of cells to collagen has been reported previously to require the presence of serum and the particular serum protein involved in this process, variously known as CIG, CAP or fibronectin, has been isolated. This conclusion that cell attachment to collagen requires serum (or more precisely, fibronectin) is based on experiments measuring the kinetics of cell attachment to films of collagen. We have measured the kinetics of attachment of HeLa and attachment to films of collagen-containing substrata under a variety of experimental conditions and present evidence that the serum-dependent mechanism of cell attachment described by others is actually only the case for films of denatured collagen, while cell attachment to native collagen fibres occurs by a different, serum-independent, mechanism. The possible relevance of these findings to cell-matrix interactions in vivo is discussed.


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