Show simple item record

dc.contributor.authorAllen, Terence D
dc.contributor.authorIype, P
dc.date.accessioned2011-07-15T11:30:01Z
dc.date.available2011-07-15T11:30:01Z
dc.date.issued1979
dc.identifier.citationUltrastructural morphology of three-dimensional colonies of cells derived from a hepatocellular carcinoma. 1979, 95 (3):225-32 J Cancer Res Clin Oncolen
dc.identifier.issn0171-5216
dc.identifier.pmid528563
dc.identifier.doi10.1007/BF00410643
dc.identifier.urihttp://hdl.handle.net/10541/136150
dc.description.abstractCultured hepatocellular carcinoma cells were studied during anchorage-independent growth in semi solid medium (Methocel). The regular occurrence of mitotic figures both at the surface and within the colonies precludes the possibility of such colonies being formed by re-aggregation. The estimated population doubling time in the three-dimensional (3-D) colonies is consistent with those two-dimensional of (2-D) colonies. Structures resembling bile canaliculi were observed between the closely opposed membranes from the well packed adjacent cells. Cell surface and ultrastructural features of the colonies and individual cells are presented and comparisons made with 2-D growth of normal and malignant liver cells in vitro. The formation of 3-D colonies may not only be an assay for transformed cells but also for predicting the type of tumors produced by re-innoculation of the in vitro transformed cells.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshCell Division
dc.subject.meshCell Transformation, Neoplastic
dc.subject.meshCells, Cultured
dc.subject.meshClone Cells
dc.subject.meshIntercellular Junctions
dc.subject.meshLiver Neoplasms, Experimental
dc.subject.meshMicroscopy, Electron, Scanning
dc.subject.meshRats
dc.titleUltrastructural morphology of three-dimensional colonies of cells derived from a hepatocellular carcinoma.en
dc.typeArticleen
dc.contributor.departmentPaterson Laboratories, Christie Hospital and Holt Radium Institute, Manchesteren
dc.identifier.journalJournal of Cancer Research and Clinical Oncologyen
html.description.abstractCultured hepatocellular carcinoma cells were studied during anchorage-independent growth in semi solid medium (Methocel). The regular occurrence of mitotic figures both at the surface and within the colonies precludes the possibility of such colonies being formed by re-aggregation. The estimated population doubling time in the three-dimensional (3-D) colonies is consistent with those two-dimensional of (2-D) colonies. Structures resembling bile canaliculi were observed between the closely opposed membranes from the well packed adjacent cells. Cell surface and ultrastructural features of the colonies and individual cells are presented and comparisons made with 2-D growth of normal and malignant liver cells in vitro. The formation of 3-D colonies may not only be an assay for transformed cells but also for predicting the type of tumors produced by re-innoculation of the in vitro transformed cells.


This item appears in the following Collection(s)

Show simple item record