Ultrastructural morphology of three-dimensional colonies of cells derived from a hepatocellular carcinoma.
Affiliation
Paterson Laboratories, Christie Hospital and Holt Radium Institute, ManchesterIssue Date
1979
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Cultured hepatocellular carcinoma cells were studied during anchorage-independent growth in semi solid medium (Methocel). The regular occurrence of mitotic figures both at the surface and within the colonies precludes the possibility of such colonies being formed by re-aggregation. The estimated population doubling time in the three-dimensional (3-D) colonies is consistent with those two-dimensional of (2-D) colonies. Structures resembling bile canaliculi were observed between the closely opposed membranes from the well packed adjacent cells. Cell surface and ultrastructural features of the colonies and individual cells are presented and comparisons made with 2-D growth of normal and malignant liver cells in vitro. The formation of 3-D colonies may not only be an assay for transformed cells but also for predicting the type of tumors produced by re-innoculation of the in vitro transformed cells.Citation
Ultrastructural morphology of three-dimensional colonies of cells derived from a hepatocellular carcinoma. 1979, 95 (3):225-32 J Cancer Res Clin OncolJournal
Journal of Cancer Research and Clinical OncologyDOI
10.1007/BF00410643PubMed ID
528563Type
ArticleLanguage
enISSN
0171-5216ae974a485f413a2113503eed53cd6c53
10.1007/BF00410643
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