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dc.contributor.authorGarland, J M
dc.contributor.authorDexter, T Michael
dc.date.accessioned2011-07-13T11:33:41Z
dc.date.available2011-07-13T11:33:41Z
dc.date.issued1981
dc.identifier.citationStudies on the in vitro culture of murine fetal thymus: effect of factors promoting epithelial growth. 1981, 1 (3):129-39 Am J Reprod Immunolen
dc.identifier.issn0271-7352
dc.identifier.pmid6461267
dc.identifier.urihttp://hdl.handle.net/10541/135957
dc.description.abstractFourteen-day fetal thymus was grown in organ culture under conditions reportedly optimizing epidermal/epithelial cell growth. While the fibroblastoid component is stimulated by epidermal growth factor and fibroblastoid growth factor, embryonic thymic epithelial cells do not grow significantly under any conditions, although they persist in organ explants. The major cell types cultured are fibroblastoid and macrophage. In organ explant cultures, epithelial cells appear to be oriented by the presence of a cell coat demonstrated by electron microscopy. This coat is absent from certain ciliated cells consistently found in deeper regions of the explant. Using amniotic fluid as a growth factor, almost pure cultures of thymic macrophages can be obtained.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshCalcium
dc.subject.meshCell Division
dc.subject.meshEpidermal Growth Factor
dc.subject.meshEpithelial Cells
dc.subject.meshFemale
dc.subject.meshFibroblast Growth Factors
dc.subject.meshFibroblasts
dc.subject.meshMacrophages
dc.subject.meshMice
dc.subject.meshMicroscopy, Electron
dc.subject.meshOrgan Culture Techniques
dc.subject.meshPeptides
dc.subject.meshPregnancy
dc.subject.meshThymus Gland
dc.titleStudies on the in vitro culture of murine fetal thymus: effect of factors promoting epithelial growth.en
dc.typeArticleen
dc.identifier.journalAmerican Journal of Reproductive Immunologyen
html.description.abstractFourteen-day fetal thymus was grown in organ culture under conditions reportedly optimizing epidermal/epithelial cell growth. While the fibroblastoid component is stimulated by epidermal growth factor and fibroblastoid growth factor, embryonic thymic epithelial cells do not grow significantly under any conditions, although they persist in organ explants. The major cell types cultured are fibroblastoid and macrophage. In organ explant cultures, epithelial cells appear to be oriented by the presence of a cell coat demonstrated by electron microscopy. This coat is absent from certain ciliated cells consistently found in deeper regions of the explant. Using amniotic fluid as a growth factor, almost pure cultures of thymic macrophages can be obtained.


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