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dc.contributor.authorXu, Cheng Xiong
dc.contributor.authorHendry, Jolyon H
dc.date.accessioned2011-07-12T17:33:37Z
dc.date.available2011-07-12T17:33:37Z
dc.date.issued1981-09
dc.identifier.citationThe radial distribution of fibroblastic colony-forming units in mouse femoral marrow. 1981, 35 (4):119-22 Biomedicineen
dc.identifier.issn0300-0893
dc.identifier.pmid7295861
dc.identifier.urihttp://hdl.handle.net/10541/135903
dc.description.abstractThe distribution of fibroblastic colony forming units (CFU-F) in mouse femoral marrow was investigated using a method which separates bone marrow cells into two regional fractions of varying size. It is shown that the concentration of CFU-F decreases almost linearly from the femoral axis (800-1,000 CFU-F per 10(7) bone marrow cells) to the bone surface (300 CFU-F per 10(7) cells). When irradiated axial or marginal cells were added into cultures as feeder cells, the colony numbers produced by both axial or marginal CFU-F were increased by about 50% and the higher concentration in the axial regions remained. The survival curve for CFU-F in both regions was characterised by a Do value of 1.54 +/- 0.11 Gy and an extrapolation number of 2.60 +/- 0.38. The results are compared to the distributions of haemopoietic stem cells and committed progenitor cells.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshBone Marrow Cells
dc.subject.meshColony-Forming Units Assay
dc.subject.meshFemur
dc.subject.meshFibroblasts
dc.subject.meshMale
dc.subject.meshMice
dc.titleThe radial distribution of fibroblastic colony-forming units in mouse femoral marrow.en
dc.typeArticleen
dc.identifier.journalBiomedicineen
html.description.abstractThe distribution of fibroblastic colony forming units (CFU-F) in mouse femoral marrow was investigated using a method which separates bone marrow cells into two regional fractions of varying size. It is shown that the concentration of CFU-F decreases almost linearly from the femoral axis (800-1,000 CFU-F per 10(7) bone marrow cells) to the bone surface (300 CFU-F per 10(7) cells). When irradiated axial or marginal cells were added into cultures as feeder cells, the colony numbers produced by both axial or marginal CFU-F were increased by about 50% and the higher concentration in the axial regions remained. The survival curve for CFU-F in both regions was characterised by a Do value of 1.54 +/- 0.11 Gy and an extrapolation number of 2.60 +/- 0.38. The results are compared to the distributions of haemopoietic stem cells and committed progenitor cells.


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