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dc.contributor.authorHall, Janet A
dc.contributor.authorSaffhill, Roy
dc.contributor.authorGreen, T
dc.contributor.authorHathway, D E
dc.date.accessioned2011-07-12T17:29:27Z
dc.date.available2011-07-12T17:29:27Z
dc.date.issued1981
dc.identifier.citationThe induction of errors during in vitro DNA synthesis following chloroacetaldehyde-treatment of poly(dA-dT) and poly(dC-dG) templates. 1981, 2 (2):141-6 Carcinogenesisen
dc.identifier.issn0143-3334
dc.identifier.pmid7023722
dc.identifier.doi10.1093/carcin/2.2.141
dc.identifier.urihttp://hdl.handle.net/10541/135902
dc.description.abstractChloroacetaldehyde, a rearranged metabolic product of the human carcinogen vinyl chloride, reacts with the DNA-like polymers poly(dA-dT) and poly(dC-dG) to form etheno-adducts of the adenine and cytosine bases. These treated polymers, when used as templates for E. coli DNA polymerase I in an in vitro assay, show a decreased ability to direct DNA synthesis. At the same time, increased relative levels of non-complementary nucleotides are incorporated. With the poly(dA-dT) templates 1 dGMP residue is incorporated for every approx 60 ethenoadenine residues present whilst no increased misincorporation of dCMP was detected. With the poly(dC-dG) templates 1 misincorporation of dAMP or dTMP occurred in the presence of approx 30 and 80 ethenocytosine residues respectively. A nearest neighbour analysis shows that with the modified poly(dC-dG) templates the majority of the errors were incorporated opposite cytosine (or modified cytosine) bases.
dc.language.isoenen
dc.subject.meshAcetaldehyde
dc.subject.meshDNA
dc.subject.meshDNA Polymerase I
dc.subject.meshEscherichia coli
dc.subject.meshPoly dA-dT
dc.subject.meshPolydeoxyribonucleotides
dc.subject.meshTemplates, Genetic
dc.titleThe induction of errors during in vitro DNA synthesis following chloroacetaldehyde-treatment of poly(dA-dT) and poly(dC-dG) templates.en
dc.typeArticleen
dc.contributor.departmentPaterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BXen
dc.identifier.journalCarcinogenesisen
html.description.abstractChloroacetaldehyde, a rearranged metabolic product of the human carcinogen vinyl chloride, reacts with the DNA-like polymers poly(dA-dT) and poly(dC-dG) to form etheno-adducts of the adenine and cytosine bases. These treated polymers, when used as templates for E. coli DNA polymerase I in an in vitro assay, show a decreased ability to direct DNA synthesis. At the same time, increased relative levels of non-complementary nucleotides are incorporated. With the poly(dA-dT) templates 1 dGMP residue is incorporated for every approx 60 ethenoadenine residues present whilst no increased misincorporation of dCMP was detected. With the poly(dC-dG) templates 1 misincorporation of dAMP or dTMP occurred in the presence of approx 30 and 80 ethenocytosine residues respectively. A nearest neighbour analysis shows that with the modified poly(dC-dG) templates the majority of the errors were incorporated opposite cytosine (or modified cytosine) bases.


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