The induction of errors during in vitro DNA synthesis following chloroacetaldehyde-treatment of poly(dA-dT) and poly(dC-dG) templates.
| dc.contributor.author | Hall, Janet A | |
| dc.contributor.author | Saffhill, Roy | |
| dc.contributor.author | Green, T | |
| dc.contributor.author | Hathway, D E | |
| dc.date.accessioned | 2011-07-12T17:29:27Z | |
| dc.date.available | 2011-07-12T17:29:27Z | |
| dc.date.issued | 1981 | |
| dc.identifier.citation | The induction of errors during in vitro DNA synthesis following chloroacetaldehyde-treatment of poly(dA-dT) and poly(dC-dG) templates. 1981, 2 (2):141-6 Carcinogenesis | en |
| dc.identifier.issn | 0143-3334 | |
| dc.identifier.pmid | 7023722 | |
| dc.identifier.doi | 10.1093/carcin/2.2.141 | |
| dc.identifier.uri | http://hdl.handle.net/10541/135902 | |
| dc.description.abstract | Chloroacetaldehyde, a rearranged metabolic product of the human carcinogen vinyl chloride, reacts with the DNA-like polymers poly(dA-dT) and poly(dC-dG) to form etheno-adducts of the adenine and cytosine bases. These treated polymers, when used as templates for E. coli DNA polymerase I in an in vitro assay, show a decreased ability to direct DNA synthesis. At the same time, increased relative levels of non-complementary nucleotides are incorporated. With the poly(dA-dT) templates 1 dGMP residue is incorporated for every approx 60 ethenoadenine residues present whilst no increased misincorporation of dCMP was detected. With the poly(dC-dG) templates 1 misincorporation of dAMP or dTMP occurred in the presence of approx 30 and 80 ethenocytosine residues respectively. A nearest neighbour analysis shows that with the modified poly(dC-dG) templates the majority of the errors were incorporated opposite cytosine (or modified cytosine) bases. | |
| dc.language.iso | en | en |
| dc.subject.mesh | Acetaldehyde | |
| dc.subject.mesh | DNA | |
| dc.subject.mesh | DNA Polymerase I | |
| dc.subject.mesh | Escherichia coli | |
| dc.subject.mesh | Poly dA-dT | |
| dc.subject.mesh | Polydeoxyribonucleotides | |
| dc.subject.mesh | Templates, Genetic | |
| dc.title | The induction of errors during in vitro DNA synthesis following chloroacetaldehyde-treatment of poly(dA-dT) and poly(dC-dG) templates. | en |
| dc.type | Article | en |
| dc.contributor.department | Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BX | en |
| dc.identifier.journal | Carcinogenesis | en |
| html.description.abstract | Chloroacetaldehyde, a rearranged metabolic product of the human carcinogen vinyl chloride, reacts with the DNA-like polymers poly(dA-dT) and poly(dC-dG) to form etheno-adducts of the adenine and cytosine bases. These treated polymers, when used as templates for E. coli DNA polymerase I in an in vitro assay, show a decreased ability to direct DNA synthesis. At the same time, increased relative levels of non-complementary nucleotides are incorporated. With the poly(dA-dT) templates 1 dGMP residue is incorporated for every approx 60 ethenoadenine residues present whilst no increased misincorporation of dCMP was detected. With the poly(dC-dG) templates 1 misincorporation of dAMP or dTMP occurred in the presence of approx 30 and 80 ethenocytosine residues respectively. A nearest neighbour analysis shows that with the modified poly(dC-dG) templates the majority of the errors were incorporated opposite cytosine (or modified cytosine) bases. |