Quantitative development of adherent cell colonies in bone marrow cell culture in vitro.
MetadataShow full item record
AbstractQuantification of the formation of adherent cell colonies in bone marrow cell culture was attempted. By secondary transfer of the bone marrow cells as a single cell suspension after 4 days' culture of fine marrow fragments, a linear relationship was obtained between the number of adherent cell colonies developing and the number of cells secondarily inoculated into the culture bottle. This suggests that 4 days' culture of the bone marrow cells with close intercellular interactions is sufficient for the 'conditioning' of the cells to develop adherent cell colonies. Activity of such colonies to support haemopoietic stem cell proliferation was also shown.
CitationQuantitative development of adherent cell colonies in bone marrow cell culture in vitro. 1979, 7 (4):171-6 Exp Hematol
- The effects of incubation of marrow in tissue fragments in vitro on the growth of adherent cells from this marrow.
- Authors: Ptasznik A, Wiktor-Jedrzejczak W
- Issue date: 1983
- In vitro proliferation of haemopoietic cells in the presence of adherent cell layers. I. Culture conditions and strain dependence.
- Authors: Reimann J, Burger H
- Issue date: 1979 Jan
- [The humoral nature of the colony-stimulating action of bone marrow cells on stromal colony formation in bone marrow cultures].
- Authors: Fridenshteĭn AIa, Latsinik NV, Gorskaia IuF, Luriia EA
- Issue date: 1990 Nov
- Cycle initiation and colony formation in culture by murine marrow cells with long-term reconstituting potential in vivo.
- Authors: Trevisan M, Yan XQ, Iscove NN
- Issue date: 1996 Dec 1
- Conditions of adherent cell growth from murine bone marrow in liquid cultures, and partial characterization of function.
- Authors: Wiktor-Jedrzejczak W, Ahmed A, Szczylik C
- Issue date: 1981 Sep