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    Quantitative development of adherent cell colonies in bone marrow cell culture in vitro.

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    Authors
    Mori, K
    Fujitake, H
    Okubo, H
    Dexter, T Michael
    Ito, Y
    Issue Date
    1979-04
    
    Metadata
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    Abstract
    Quantification of the formation of adherent cell colonies in bone marrow cell culture was attempted. By secondary transfer of the bone marrow cells as a single cell suspension after 4 days' culture of fine marrow fragments, a linear relationship was obtained between the number of adherent cell colonies developing and the number of cells secondarily inoculated into the culture bottle. This suggests that 4 days' culture of the bone marrow cells with close intercellular interactions is sufficient for the 'conditioning' of the cells to develop adherent cell colonies. Activity of such colonies to support haemopoietic stem cell proliferation was also shown.
    Citation
    Quantitative development of adherent cell colonies in bone marrow cell culture in vitro. 1979, 7 (4):171-6 Exp Hematol
    Journal
    Experimental Hematology
    URI
    http://hdl.handle.net/10541/135854
    PubMed ID
    477767
    Type
    Article
    Language
    en
    ISSN
    0301-472X
    Collections
    All Paterson Institute for Cancer Research

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