Involvement of mitochondria in changes in fluorescein excitation and emission polarization spectra in living cells.
Affiliation
Paterson Laboratories, Christie Hospital and Holt Radium Institute, ManchesterIssue Date
1979-12
Metadata
Show full item recordAbstract
The comparison of fluorescein polarization spectra in living cells and in isolated subcellular structures identified the mitochondria as the cytoplasmic domain in which on excitation at 470 nm the sharp fluorescein emission polarization peak at 510 nm is formed. Changes in the emission polarization peak during the cell cycle or those induced by growth stimulators and inhibitors reflect structural changes in the mitochondria on their transition from the resting, orthodox into the active, ATP-generating, condensed conformation and vice versa. Possible mechanisms for the formation of the sharp emission polarization peak are discussed.Citation
Involvement of mitochondria in changes in fluorescein excitation and emission polarization spectra in living cells. 1979, 28 (3):403-11 Biophys JJournal
Biophysical JournalDOI
10.1016/S0006-3495(79)85189-9PubMed ID
262558Type
ArticleLanguage
enISSN
0006-3495ae974a485f413a2113503eed53cd6c53
10.1016/S0006-3495(79)85189-9