• Login
    View Item 
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ChristieCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsProfilesView

    My Account

    LoginRegister

    Local Links

    The Christie WebsiteChristie Library and Knowledge Service

    Statistics

    Display statistics

    Systemic and in-situ natural killer activity in tumour-bearing rats.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Moore, K
    Moore, Michael
    Issue Date
    1979-06
    
    Metadata
    Show full item record
    Abstract
    Single-cell suspensions prepared by enzymatic disaggregation of an immunogenic 3-methylcholanthrene-induced sarcoma (Mc40A) contain a significant proportion of infiltrating leucocytes (approximately 42%), comprising T lymphocytes, macrophages and non-phagocytic FcR+ lymphoid-like cells. Tumour-infiltrating lymphocytes (TIL) were isolated and purified by successive passage over Sephadex G-10 columns and their cytotoxic activity in vitro compared with that of lymphoid cells from normal rats and from tumour-bearers at different times after implantation. For this purpose, surviving target cells were quantified by incorporation of the gamma-emitting analogue of methionine, 75Sel-methionine, in a 48-h assay which detected both cytotoxic and cytostatic effects. The reactivity of TIL, which was consistently demonstrable from 11 days after tumour transplantation, was essentially similar to that of normal splenic lymphocytes in magnitude and specificity. Reciprocal cytotoxicity tests using TIL and cultured targets from an antigenically unrelated tumour of similar aetiology (Mc57) showed that the manifestation of TIL cytotoxicity was determined, not by the tumour of origin, but by the susceptibility of the target cells. Evidence that the effector function of TIL was mediated in part by natural killer (NK) cells was derived from concurrent experiments using human myeloid cells (K562) as targets in an 18h 51Cr-release assay. In this system the level of NK activity was critically dependent on the numbers of tumour cells in the TIL population; contamination in excess of 2% gave rise to dose-dependent inhibition of NK function. The results show that within a progressively growing tumour known to possess rejection antigens, NK reactivity was detected in the absence of a demonstrable tumour-specific cytotoxic component.
    Citation
    Systemic and in-situ natural killer activity in tumour-bearing rats. 1979, 39 (6):636-47 Br J Cancer
    Journal
    British Journal of Cancer
    URI
    http://hdl.handle.net/10541/135774
    PubMed ID
    375965
    Type
    Article
    Language
    en
    ISSN
    0007-0920
    Collections
    All Paterson Institute for Cancer Research

    entitlement

    Related articles

    • Recombinant tumor necrosis factor alpha inhibits growth of methylcholanthrene-induced sarcoma and enhances natural killer activity of tumor-infiltrating lymphocytes in aging rats.
    • Authors: Ziółkowska M, Nowak JJ, Janiak M, Ryzewska A
    • Issue date: 1993
    • Evaluation of 51Cr release for detecting cell-mediated cytotoxic responses to solid chemically induced rat tumours.
    • Authors: Zöller M, Price MR, Baldwin RW
    • Issue date: 1977 Jun
    • Variable natural killer function of tumour-infiltrating lymphocytes from breast carcinomas.
    • Authors: Gudmundsdóttir I, Ogmundsdóttir HM
    • Issue date: 1992 Aug
    • Immunity to chemically induced rat sarcomas: study of the specificity of cytotoxic cells.
    • Authors: Goguel AF, Nauciel C
    • Issue date: 1979 Sep-Oct
    • Extravascular natural cytotoxicity in man: Anti-K562 activity of lymph-node and tumour-infiltrating lymphocytes.
    • Authors: Moore M, Vose BM
    • Issue date: 1981 Mar 15
    DSpace software (copyright © 2002 - 2025)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.