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dc.contributor.authorMoore, James V
dc.date.accessioned2011-07-11T08:36:28Z
dc.date.available2011-07-11T08:36:28Z
dc.date.issued1979-02
dc.identifier.citationAblation of murine jejunal crypts by alkylating agents. 1979, 39 (2):175-81 Br J Canceren
dc.identifier.issn0007-0920
dc.identifier.pmid219878
dc.identifier.urihttp://hdl.handle.net/10541/135773
dc.description.abstractThe gut microcolony assay has been used to measure damage to intestinal crypts by single and split doses of 3 alkylating agents: mechlorethamine hydrochloride (HN2), bis-chloroethyl-nitrosourea (BCNU) and isopropyl methane sulphonate (IMS). The single-dose survival curves for whole crypts were distinguished by extrapolation numbers (3.0, 176 and 1.5 respectively) that were lower than most previously published values for assay by irradiation. Significant sparing of crypts occurred when doses of HN2 or BCNU, but not IMS, were given in 2 equal fractions separated by more than 2 h. Deduced D0 values for those cells from which crypts regenerate were 1.9 mg/kg HN2, 19 mg/kg BCNU and 487 mg/kg IMS.
dc.language.isoenen
dc.subject.meshAlkylating Agents
dc.subject.meshAnimals
dc.subject.meshCarmustine
dc.subject.meshCell Survival
dc.subject.meshDose-Response Relationship, Drug
dc.subject.meshJejunum
dc.subject.meshMale
dc.subject.meshMechlorethamine
dc.subject.meshMesylates
dc.subject.meshMice
dc.titleAblation of murine jejunal crypts by alkylating agents.en
dc.typeArticleen
dc.contributor.departmentPaterson Laboratories, Christie Hospital and Holt Radium Institute, Manchesteren
dc.identifier.journalBritish Journal of Canceren
html.description.abstractThe gut microcolony assay has been used to measure damage to intestinal crypts by single and split doses of 3 alkylating agents: mechlorethamine hydrochloride (HN2), bis-chloroethyl-nitrosourea (BCNU) and isopropyl methane sulphonate (IMS). The single-dose survival curves for whole crypts were distinguished by extrapolation numbers (3.0, 176 and 1.5 respectively) that were lower than most previously published values for assay by irradiation. Significant sparing of crypts occurred when doses of HN2 or BCNU, but not IMS, were given in 2 equal fractions separated by more than 2 h. Deduced D0 values for those cells from which crypts regenerate were 1.9 mg/kg HN2, 19 mg/kg BCNU and 487 mg/kg IMS.


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