The role of cells and their products in the regulation of in vitro stem cell proliferation and granulocyte development.
AffiliationPaterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BX, England
MetadataShow full item record
AbstractIn long-term marrow cultures haemopoiesis can be maintained in vitro for up to 6 months. Critical analysis of the cell populations produced has shown that the stem cells and their committed progeny have characteristics in common with the corresponding cell types in vivo. The maintenance of haemopoiesis in vitro is associated with the development of an appropriate inductive environment provided by bone marrow derived adherent cells. Analysis of the interactions between environmental and haemopoietic cells has been facilitated by the development of vitro systems reproducing the naturally occurring genetic environmental defects and other systems where the development of a competent inductive environment shows a dependency upon corticosteroid hormones. Investigations have shown that stem cell proliferation may be controlled by production of opposing activities, one stimulatory for DNA synthesis, the other inhibitory. A model is proposed whereby modulation in the production of these factors is determined by the physical presence of stem cells in a proposed cellular milieu, within the adherent layer. The adherent layer, apart from acting at the level of stem cell proliferation, can also modify the response of differentiating cells (eg, GM-CFC) to exogenous stimulatory activities. Addition of GM-CSF or of CSF-antiserum has no effect on haemopoiesis in long-term cultures.
CitationThe role of cells and their products in the regulation of in vitro stem cell proliferation and granulocyte development. 1980, 13 (4):513-24 J Supramol Struct
JournalJournal of Supramolecular Structure
- Long term bone marrow cultures: an ultrastructural review.
- Authors: Allen TD, Dexter TM
- Issue date: 1983
- Cloning and frequency analysis of haemopoietic stem cells producing CFC over weeks in long-term marrow cultures.
- Authors: Mergenthaler HG, Staber FG, Dörmer P
- Issue date: 1983 Jul
- The regulation of haemopoiesis in long-term bone marrow cultures: I. role of L-cell CSF.
- Authors: Dexter TM, Shadduck RK
- Issue date: 1980 Mar
- Effect of stem cell factor (c-kit ligand), granulocyte-macrophage colony stimulating factor and interleukin 3 on hematopoietic progenitors in human long-term bone marrow cultures.
- Authors: Lemoli RM, Gulati SC
- Issue date: 1993 Sep