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    The role of cells and their products in the regulation of in vitro stem cell proliferation and granulocyte development.

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    Authors
    Dexter, T Michael
    Spooncer, Elaine
    Toksoz, D
    Lajtha, L G
    Affiliation
    Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BX, England
    Issue Date
    1980
    
    Metadata
    Show full item record
    Abstract
    In long-term marrow cultures haemopoiesis can be maintained in vitro for up to 6 months. Critical analysis of the cell populations produced has shown that the stem cells and their committed progeny have characteristics in common with the corresponding cell types in vivo. The maintenance of haemopoiesis in vitro is associated with the development of an appropriate inductive environment provided by bone marrow derived adherent cells. Analysis of the interactions between environmental and haemopoietic cells has been facilitated by the development of vitro systems reproducing the naturally occurring genetic environmental defects and other systems where the development of a competent inductive environment shows a dependency upon corticosteroid hormones. Investigations have shown that stem cell proliferation may be controlled by production of opposing activities, one stimulatory for DNA synthesis, the other inhibitory. A model is proposed whereby modulation in the production of these factors is determined by the physical presence of stem cells in a proposed cellular milieu, within the adherent layer. The adherent layer, apart from acting at the level of stem cell proliferation, can also modify the response of differentiating cells (eg, GM-CFC) to exogenous stimulatory activities. Addition of GM-CSF or of CSF-antiserum has no effect on haemopoiesis in long-term cultures.
    Citation
    The role of cells and their products in the regulation of in vitro stem cell proliferation and granulocyte development. 1980, 13 (4):513-24 J Supramol Struct
    Journal
    Journal of Supramolecular Structure
    URI
    http://hdl.handle.net/10541/135758
    DOI
    10.1002/jss.400130410
    PubMed ID
    7230803
    Type
    Article
    Language
    en
    ISSN
    0091-7419
    ae974a485f413a2113503eed53cd6c53
    10.1002/jss.400130410
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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