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dc.contributor.authorDexter, T Michael
dc.date.accessioned2011-07-11T09:08:20Z
dc.date.available2011-07-11T09:08:20Z
dc.date.issued1979
dc.identifier.citationHaemopoiesis in long-term bone marrow cultures. A review. 1979, 62 (5-6):299-305 Acta Haematolen
dc.identifier.issn0001-5792
dc.identifier.pmid121673
dc.identifier.doi10.1159/000207593
dc.identifier.urihttp://hdl.handle.net/10541/135756
dc.description.abstractBone marrow-derived adherent cell layers, containing endothelial cells, fat cells and macrophages will support prolonged haemopoiesis in vitro. Evidence suggests that the adherent layer is acting as an in vitro haemopoietic inductive environment for stem cell proliferation and differentiation into the variety of committed progenitor cells of the granulocytic, erythroid, megakaryocytic and lymphoid lineages. Using this system we have analysed the factors controlling proliferation of stem cells (CFU-S), differentiation and maturation of granulocyte/macrophage precursor cells (CFU-C), leukaemic transformation by chemicals and viruses, the role of environment versus stem cells in the aetiology of haemopoietic aplasias and the possible usefulness of long-term cultures for bone marrow transplantation.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshBone Marrow Cells
dc.subject.meshBone Marrow Diseases
dc.subject.meshBone Marrow Transplantation
dc.subject.meshCell Adhesion
dc.subject.meshCell Differentiation
dc.subject.meshCell Transformation, Neoplastic
dc.subject.meshColony-Forming Units Assay
dc.subject.meshCulture Techniques
dc.subject.meshGranulocytes
dc.subject.meshHematopoiesis
dc.subject.meshHematopoietic Stem Cells
dc.subject.meshHumans
dc.subject.meshLeukemia, Experimental
dc.subject.meshMacrophages
dc.subject.meshMice
dc.titleHaemopoiesis in long-term bone marrow cultures. A review.en
dc.typeArticleen
dc.contributor.departmentPaterson Laboratories, Christie Hospital and Holt Radium Institute, Manchesteren
dc.identifier.journalActa Haematologicaen
html.description.abstractBone marrow-derived adherent cell layers, containing endothelial cells, fat cells and macrophages will support prolonged haemopoiesis in vitro. Evidence suggests that the adherent layer is acting as an in vitro haemopoietic inductive environment for stem cell proliferation and differentiation into the variety of committed progenitor cells of the granulocytic, erythroid, megakaryocytic and lymphoid lineages. Using this system we have analysed the factors controlling proliferation of stem cells (CFU-S), differentiation and maturation of granulocyte/macrophage precursor cells (CFU-C), leukaemic transformation by chemicals and viruses, the role of environment versus stem cells in the aetiology of haemopoietic aplasias and the possible usefulness of long-term cultures for bone marrow transplantation.


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