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dc.contributor.authorVose, Brent M
dc.contributor.authorMoore, Michael
dc.date.accessioned2011-07-07T11:14:07Z
dc.date.available2011-07-07T11:14:07Z
dc.date.issued1980-08
dc.identifier.citationNatural cytotoxicity in humans: susceptibility of freshly isolatd tumor cells to lysis. 1980, 65 (2):257-63 J Natl Cancer Insten
dc.identifier.issn0027-8874
dc.identifier.pmid6157044
dc.identifier.urihttp://hdl.handle.net/10541/135532
dc.description.abstractThe cytotoxic potential of blood lymphocyters from healthy donors was tested against freshly isolated lung cancer cells and the erythroleukemia K562 cell line in short-term 51Cr release assays conducted at an effector:target ratio of 50:1. Most donors exhibited significant activity against K6-562 cells. By contrast, fresh tumor cells were refractory, only 6 of 30 showing significant cytotoxicity. The low susceptibility of these tumor cells was confirmed in third-party cold inhibition assays in which they interfered minimally with killing of K562 targets under conditions in which unlabeled K562 cells efficiently blocked cytotoxicity. Cells prepared from normal lung tissue and Raji cells also failed to inhibit killing. Although in comparison to the K562 cell line freshly isolated tumor cells were resistant, their susceptibility may not be so low as to be biologically irrelevant, inasmuch as boosting of natural killing activity by interferon induced levels of cytotoxicity against both types of target cell that were unattainable by unstimulated effectors. Interferon-boosted killers were lytic for "normal" lung cells and the Raji cell line.
dc.language.isoenen
dc.subject.meshCell Line
dc.subject.meshCytotoxicity, Immunologic
dc.subject.meshHumans
dc.subject.meshImmunity, Innate
dc.subject.meshInterferons
dc.subject.meshLeukemia, Erythroblastic, Acute
dc.subject.meshLung Neoplasms
dc.subject.meshLymphocytes
dc.titleNatural cytotoxicity in humans: susceptibility of freshly isolatd tumor cells to lysis.en
dc.typeArticleen
dc.contributor.departmentPaterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BX, Englanden
dc.identifier.journalJournal of the National Cancer Instituteen
html.description.abstractThe cytotoxic potential of blood lymphocyters from healthy donors was tested against freshly isolated lung cancer cells and the erythroleukemia K562 cell line in short-term 51Cr release assays conducted at an effector:target ratio of 50:1. Most donors exhibited significant activity against K6-562 cells. By contrast, fresh tumor cells were refractory, only 6 of 30 showing significant cytotoxicity. The low susceptibility of these tumor cells was confirmed in third-party cold inhibition assays in which they interfered minimally with killing of K562 targets under conditions in which unlabeled K562 cells efficiently blocked cytotoxicity. Cells prepared from normal lung tissue and Raji cells also failed to inhibit killing. Although in comparison to the K562 cell line freshly isolated tumor cells were resistant, their susceptibility may not be so low as to be biologically irrelevant, inasmuch as boosting of natural killing activity by interferon induced levels of cytotoxicity against both types of target cell that were unattainable by unstimulated effectors. Interferon-boosted killers were lytic for "normal" lung cells and the Raji cell line.


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