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dc.contributor.authorScolnick, Een
dc.contributor.authorWeeks, Men
dc.contributor.authorShih, Ten
dc.contributor.authorRuscetti, Sen
dc.contributor.authorDexter, T Michaelen
dc.date.accessioned2011-07-01T12:01:56Z
dc.date.available2011-07-01T12:01:56Z
dc.date.issued1981-01
dc.identifier.citationMarkedly elevated levels of an endogenous sarc protein in a hemopoietic precursor cell line. 1981, 1 (1):66-74 Mol Cell Biolen
dc.identifier.issn0270-7306
dc.identifier.pmid6821513
dc.identifier.urihttp://hdl.handle.net/10541/135058
dc.description.abstractThe src gene product of Harvey murine sarcoma virus is a 21,000-dalton guanine nucleotide-binding protein. We have recently shown that a wide variety of vertebrate cell strains and cell lines express much lower levels of an endogenous p21 immunologically related to the Harvey murine sarcoma virus-coded p21. In this report, we have examined the levels of endogenous p21 in a unique hemopoietic precursor cell line, 416B, which was originally described as a continuous cell line of a hemopoietic stem cell, CFU-S. The currently available 416B cells express markedly elevated levels of endogenous p21. The level of endogenous p21 in the 416B cells is 5- to 10-fold higher than the level of p21 in Harvey murine sarcoma virus-infected cells and more than 100 times higher than the level of endogenous p21 that we have observed in a variety of other fresh or cultured cells. The results indicate that marked regulation of the levels of an endogenous sarc gene product can occur, and speculation about a possible role for endogenous p21 in normal hemopoietic stem cells is discussed.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshCell Line, Transformed
dc.subject.meshDNA, Viral
dc.subject.meshGTP-Binding Proteins
dc.subject.meshGenes, Viral
dc.subject.meshHarvey murine sarcoma virus
dc.subject.meshHematopoietic Stem Cells
dc.subject.meshOncogene Protein p21(ras)
dc.subject.meshOncogene Proteins, Viral
dc.titleMarkedly elevated levels of an endogenous sarc protein in a hemopoietic precursor cell line.en
dc.typeArticleen
dc.contributor.departmentLaboratory of Tumor Virus Genetics, National Cancer Institute, Bethesda, Maryland 20205.en
dc.identifier.journalMolecular and Cellular Biologyen
html.description.abstractThe src gene product of Harvey murine sarcoma virus is a 21,000-dalton guanine nucleotide-binding protein. We have recently shown that a wide variety of vertebrate cell strains and cell lines express much lower levels of an endogenous p21 immunologically related to the Harvey murine sarcoma virus-coded p21. In this report, we have examined the levels of endogenous p21 in a unique hemopoietic precursor cell line, 416B, which was originally described as a continuous cell line of a hemopoietic stem cell, CFU-S. The currently available 416B cells express markedly elevated levels of endogenous p21. The level of endogenous p21 in the 416B cells is 5- to 10-fold higher than the level of p21 in Harvey murine sarcoma virus-infected cells and more than 100 times higher than the level of endogenous p21 that we have observed in a variety of other fresh or cultured cells. The results indicate that marked regulation of the levels of an endogenous sarc gene product can occur, and speculation about a possible role for endogenous p21 in normal hemopoietic stem cells is discussed.


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