Markedly elevated levels of an endogenous sarc protein in a hemopoietic precursor cell line.
| dc.contributor.author | Scolnick, E | |
| dc.contributor.author | Weeks, M | |
| dc.contributor.author | Shih, T | |
| dc.contributor.author | Ruscetti, S | |
| dc.contributor.author | Dexter, T Michael | |
| dc.date.accessioned | 2011-07-01T12:01:07Z | |
| dc.date.available | 2011-07-01T12:01:07Z | |
| dc.date.issued | 1981-01 | |
| dc.identifier.citation | Markedly elevated levels of an endogenous sarc protein in a hemopoietic precursor cell line. 1981, 1 (1):66-74 Mol. Cell. Biol. | en |
| dc.identifier.issn | 0270-7306 | |
| dc.identifier.pmid | 6821513 | |
| dc.identifier.uri | http://hdl.handle.net/10541/135057 | |
| dc.description.abstract | The src gene product of Harvey murine sarcoma virus is a 21,000-dalton guanine nucleotide-binding protein. We have recently shown that a wide variety of vertebrate cell strains and cell lines express much lower levels of an endogenous p21 immunologically related to the Harvey murine sarcoma virus-coded p21. In this report, we have examined the levels of endogenous p21 in a unique hemopoietic precursor cell line, 416B, which was originally described as a continuous cell line of a hemopoietic stem cell, CFU-S. The currently available 416B cells express markedly elevated levels of endogenous p21. The level of endogenous p21 in the 416B cells is 5- to 10-fold higher than the level of p21 in Harvey murine sarcoma virus-infected cells and more than 100 times higher than the level of endogenous p21 that we have observed in a variety of other fresh or cultured cells. The results indicate that marked regulation of the levels of an endogenous sarc gene product can occur, and speculation about a possible role for endogenous p21 in normal hemopoietic stem cells is discussed. | |
| dc.language.iso | en | en |
| dc.subject.mesh | Animals | |
| dc.subject.mesh | Cell Line, Transformed | |
| dc.subject.mesh | DNA, Viral | |
| dc.subject.mesh | GTP-Binding Proteins | |
| dc.subject.mesh | Genes, Viral | |
| dc.subject.mesh | Harvey murine sarcoma virus | |
| dc.subject.mesh | Hematopoietic Stem Cells | |
| dc.subject.mesh | Oncogene Protein p21(ras) | |
| dc.subject.mesh | Oncogene Proteins, Viral | |
| dc.title | Markedly elevated levels of an endogenous sarc protein in a hemopoietic precursor cell line. | en |
| dc.type | Article | en |
| dc.contributor.department | Laboratory of Tumor Virus Genetics, National Cancer Institute, Bethesda, Maryland 20205. | en |
| dc.identifier.journal | Molecular and Cellular Biology | en |
| html.description.abstract | The src gene product of Harvey murine sarcoma virus is a 21,000-dalton guanine nucleotide-binding protein. We have recently shown that a wide variety of vertebrate cell strains and cell lines express much lower levels of an endogenous p21 immunologically related to the Harvey murine sarcoma virus-coded p21. In this report, we have examined the levels of endogenous p21 in a unique hemopoietic precursor cell line, 416B, which was originally described as a continuous cell line of a hemopoietic stem cell, CFU-S. The currently available 416B cells express markedly elevated levels of endogenous p21. The level of endogenous p21 in the 416B cells is 5- to 10-fold higher than the level of p21 in Harvey murine sarcoma virus-infected cells and more than 100 times higher than the level of endogenous p21 that we have observed in a variety of other fresh or cultured cells. The results indicate that marked regulation of the levels of an endogenous sarc gene product can occur, and speculation about a possible role for endogenous p21 in normal hemopoietic stem cells is discussed. |