• Login
    View Item 
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ChristieCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjects

    My Account

    LoginRegister

    Local Links

    The Christie WebsiteChristie Library and Knowledge Service

    Statistics

    Display statistics

    Markedly elevated levels of an endogenous sarc protein in a hemopoietic precursor cell line.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Scolnick, E
    Weeks, M
    Shih, T
    Ruscetti, S
    Dexter, T Michael
    Affiliation
    Laboratory of Tumor Virus Genetics, National Cancer Institute, Bethesda, Maryland 20205.
    Issue Date
    1981-01
    
    Metadata
    Show full item record
    Abstract
    The src gene product of Harvey murine sarcoma virus is a 21,000-dalton guanine nucleotide-binding protein. We have recently shown that a wide variety of vertebrate cell strains and cell lines express much lower levels of an endogenous p21 immunologically related to the Harvey murine sarcoma virus-coded p21. In this report, we have examined the levels of endogenous p21 in a unique hemopoietic precursor cell line, 416B, which was originally described as a continuous cell line of a hemopoietic stem cell, CFU-S. The currently available 416B cells express markedly elevated levels of endogenous p21. The level of endogenous p21 in the 416B cells is 5- to 10-fold higher than the level of p21 in Harvey murine sarcoma virus-infected cells and more than 100 times higher than the level of endogenous p21 that we have observed in a variety of other fresh or cultured cells. The results indicate that marked regulation of the levels of an endogenous sarc gene product can occur, and speculation about a possible role for endogenous p21 in normal hemopoietic stem cells is discussed.
    Citation
    Markedly elevated levels of an endogenous sarc protein in a hemopoietic precursor cell line. 1981, 1 (1):66-74 Mol. Cell. Biol.
    Journal
    Molecular and Cellular Biology
    URI
    http://hdl.handle.net/10541/135057
    PubMed ID
    6821513
    Type
    Article
    Language
    en
    ISSN
    0270-7306
    Collections
    All Paterson Institute for Cancer Research

    entitlement

    Related articles

    • Monoclonal antibodies to the p21 products of the transforming gene of Harvey murine sarcoma virus and of the cellular ras gene family.
    • Authors: Furth ME, Davis LJ, Fleurdelys B, Scolnick EM
    • Issue date: 1982 Jul
    • In vitro retroviral transfer of ras genes to single hemopoietic progenitors.
    • Authors: Pharr PN, Ogawa M, Hankins WD
    • Issue date: 1987 May
    • Development and analysis of a transformation-defective mutant of Harvey murine sarcoma tk virus and its gene product.
    • Authors: Weeks MO, Hager GL, Lowe R, Scolnick EM
    • Issue date: 1985 May
    • Mouse cells contain two distinct ras gene mRNA species that can be translated into a p21 onc protein.
    • Authors: Ellis RW, DeFeo D, Furth ME, Scolnick EM
    • Issue date: 1982 Nov
    • Photoaffinity labeling with GTP of viral p21 ras protein expressed in Escherichia coli.
    • Authors: Stein RB, Robinson PS, Scolnick EM
    • Issue date: 1984 May
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.