Show simple item record

Malignant myelomonocytic cells after in vitro infection of marrow cells with Friend leukaemia virus.

dc.contributor.authorTesta, Nydia G
dc.contributor.authorDexter, T Michael
dc.contributor.authorScott, David
dc.contributor.authorTeich, N M
dc.date.accessioned2011-06-29T17:37:59Z
dc.date.available2011-06-29T17:37:59Z
dc.date.issued1980-01
dc.identifier.citationMalignant myelomonocytic cells after in vitro infection of marrow cells with Friend leukaemia virus. 1980, 41 (1):33-9 Br J Canceren
dc.identifier.issn0007-0920
dc.identifier.pmid6928787
dc.identifier.urihttp://hdl.handle.net/10541/134896
dc.description.abstractInfection of long-term BDF1 marrow cultures with Friend leukaemia virus complex (FLV) induced transformed cells with myelomonocytic characteristics, which were isolated only 14 days after the viral infection. Criteria for transformation were growth in suspension cultures and high plating efficiency in agar. The lymphatic leukaemia virus (LLV) replicates in these suspension cultures, but the spleen focus-forming virus (SFFV) component of the FLV complex has not been detected. Injection of the transformed cells into syngeneic neonatal or adult mice leads to the development of leukaemia which can be demonstrated to be of donor origin by the presence of two metacentric marker chromosomes which are also seen in the cultured cells.
dc.language.isoenen
dc.subject.meshAgar
dc.subject.meshAnimals
dc.subject.meshBone Marrow
dc.subject.meshCell Division
dc.subject.meshCell Line
dc.subject.meshCell Transformation, Neoplastic
dc.subject.meshCell Transformation, Viral
dc.subject.meshColony-Forming Units Assay
dc.subject.meshFriend murine leukemia virus
dc.subject.meshGenetic Markers
dc.subject.meshLeukemia, Experimental
dc.subject.meshLeukemia, Myeloid
dc.subject.meshMice
dc.subject.meshNeoplasm Transplantation
dc.subject.meshTime Factors
dc.subject.meshTransplantation, Isogeneic
dc.titleMalignant myelomonocytic cells after in vitro infection of marrow cells with Friend leukaemia virus.en
dc.typeArticleen
dc.contributor.departmentPaterson Laboratories, Christie Hospital and Holt Radium Institute, Manchesteren
dc.identifier.journalBritish Journal of Canceren
html.description.abstractInfection of long-term BDF1 marrow cultures with Friend leukaemia virus complex (FLV) induced transformed cells with myelomonocytic characteristics, which were isolated only 14 days after the viral infection. Criteria for transformation were growth in suspension cultures and high plating efficiency in agar. The lymphatic leukaemia virus (LLV) replicates in these suspension cultures, but the spleen focus-forming virus (SFFV) component of the FLV complex has not been detected. Injection of the transformed cells into syngeneic neonatal or adult mice leads to the development of leukaemia which can be demonstrated to be of donor origin by the presence of two metacentric marker chromosomes which are also seen in the cultured cells.


This item appears in the following Collection(s)

Show simple item record