Show simple item record

dc.contributor.authorSalet, C
dc.contributor.authorDe Sa E Melo, T M
dc.contributor.authorBensasson, R V
dc.contributor.authorLand, Edward J
dc.date.accessioned2011-06-29T17:44:19Z
dc.date.available2011-06-29T17:44:19Z
dc.date.issued1980-04-30
dc.identifier.citationPhotophysical properties of aminoethylpsoralen in presence and absence of DNA. 1980, 607 (2):379-83 Biochim Biophys Actaen
dc.identifier.issn0006-3002
dc.identifier.pmid7370270
dc.identifier.doi10.1016/0005-2787(80)90090-8
dc.identifier.urihttp://hdl.handle.net/10541/134875
dc.description.abstractSome properties of the first triplet and singlet excited states of 4'-aminomethyl-4,5',8-trimethylpsoralen have been determined by laser flash photolysis and spectrofluorimetry. In water the lowest triplet has an intrinsic lifetime of 100 microseconds and is quenched by the molecule in its ground state with a rate constant kQ = 10(9) M-1 . s-1 and by thymine with a rate cconstant kq = 2 . 10(8) M-1 . x-1. Addition of 0.5 mg/ml of calf thymus DNA causes the disappearance of the aminomethylpsoralen triplet absorption immediately after the laser flash excitation and extinguishes the fluorescence emission of a 6 . 10(-5) M solution of aminomethylpsoralen. These experiments show that the first singlet excited state which is rapidly deactivated in the presence of DNA could be the precursor of the first pyrimidine photoadduct.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshBiophysical Phenomena
dc.subject.meshBiophysics
dc.subject.meshCattle
dc.subject.meshDNA
dc.subject.meshKinetics
dc.subject.meshPhotochemistry
dc.subject.meshPsoralens
dc.subject.meshSpectrometry, Fluorescence
dc.subject.meshTrioxsalen
dc.titlePhotophysical properties of aminoethylpsoralen in presence and absence of DNA.en
dc.typeArticleen
dc.contributor.departmentLaboratoire de Biophysique, Muséum National d'Histoire Naturelle, 61, rue Buffon, 75005 Paris Franceen
dc.identifier.journalBiochimica et Biophysica Actaen
html.description.abstractSome properties of the first triplet and singlet excited states of 4'-aminomethyl-4,5',8-trimethylpsoralen have been determined by laser flash photolysis and spectrofluorimetry. In water the lowest triplet has an intrinsic lifetime of 100 microseconds and is quenched by the molecule in its ground state with a rate constant kQ = 10(9) M-1 . s-1 and by thymine with a rate cconstant kq = 2 . 10(8) M-1 . x-1. Addition of 0.5 mg/ml of calf thymus DNA causes the disappearance of the aminomethylpsoralen triplet absorption immediately after the laser flash excitation and extinguishes the fluorescence emission of a 6 . 10(-5) M solution of aminomethylpsoralen. These experiments show that the first singlet excited state which is rapidly deactivated in the presence of DNA could be the precursor of the first pyrimidine photoadduct.


Files in this item

This item appears in the following Collection(s)

Show simple item record