The incorporation of O4-methylthymidine into V79A cell DNA when present in the cell culture medium.
Affiliation
Paterson Laboratories, Christie Hospital and Holt Radium Institute Manchester, M20 9BX, UKIssue Date
1980-06
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When Chinese hamster V79A cells are cultured in the presence of O4-methyl-[6-3H]-thymidine the incorporation of this modified nucleoside into newly synthesised DNA is observed. The radioactivity incorporated has been identified as O4-methylthymidine by digesting the DNA to 3'-monophosphates with spleen phosphodiesterase followed by treatment with alkaline phosphatase to give O4-methylthymidine as the major radioactive product. The radioactivity associated with the latter co-chromatographs with authentic O4-methylthymidine in several chromatographic systems. Once incorporated the modified nucleoside appears to be rapidly removed from the DNA with half-life of 2-3 h. There is no evidence of demethylation of the O4-methylthymidine to give thymidine, in either the culture medium or within the cells once incorporated into DNA. Although the levels of incorporation observed are low, (being only 125 O4-methylthymidine residues per 10(8) thymidine residues at a modified nucleoside concentration of 10(-5) M), they may still be relevant as similar levels are apparently produced in the DNA of the cultured cells on treatment with biologically significant doses of carcinogenic alkylating agents.Citation
The incorporation of O4-methylthymidine into V79A cell DNA when present in the cell culture medium. 1980, 1 (6):487-93 CarcinogenesisJournal
CarcinogenesisDOI
10.1093/carcin/1.6.487PubMed ID
7273281Type
ArticleLanguage
enISSN
0143-3334ae974a485f413a2113503eed53cd6c53
10.1093/carcin/1.6.487
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