Show simple item record

dc.contributor.authorKimber, Ian
dc.contributor.authorMoore, Michael
dc.date.accessioned2011-06-23T13:20:14Z
dc.date.available2011-06-23T13:20:14Z
dc.date.issued1981-01
dc.identifier.citationLysis of alloantibody-sensitized human erythrocytes by peripheral blood mononuclear cells: heterogeneity of effector populations. 1981, 5 (1):41-6 J Clin Lab Immunolen
dc.identifier.issn0141-2760
dc.identifier.pmid6260947
dc.identifier.urihttp://hdl.handle.net/10541/134309
dc.description.abstractCell-mediated haemolysis of human erythrocytes (HRBC) mediated by sensitizing alloantibodies of two specificities was studied using the 51Cr release assay. Peripheral blood mononuclear cells (PBMC) were found capable fo lysing HRBC of phenotype ARh(D) + ve sensitized with either anti-D or "natural" anti-A antibodies. The characteristics of the effector population, however, were dependent upon the sensitizing antibody. HRBC sensitized with anti-A were lysed by a radioresistant, silica- and carrageenan-sensitive population which could be selectively removed by adherence. Sensitization with rhesus antibody induced cytotoxicity by a population which was radiosensitive and relatively unaffected by removal of adherent cells or by treatment with silica or carrageenan. The results demonstrate that the antigen-antibody interaction, rather than the target cell type, determines which effector cells participate in antibody-dependent cellular cytotoxicity (ADCC) against human red cells.
dc.language.isoenen
dc.subject.meshABO Blood-Group System
dc.subject.meshAnimals
dc.subject.meshCarrageenan
dc.subject.meshCattle
dc.subject.meshCell Adhesion
dc.subject.meshDose-Response Relationship, Radiation
dc.subject.meshErythrocytes
dc.subject.meshHemolysis
dc.subject.meshHumans
dc.subject.meshIsoantibodies
dc.subject.meshMonocytes
dc.subject.meshPhagocytes
dc.subject.meshRh-Hr Blood-Group System
dc.subject.meshSilicon Dioxide
dc.titleLysis of alloantibody-sensitized human erythrocytes by peripheral blood mononuclear cells: heterogeneity of effector populations.en
dc.typeArticleen
dc.identifier.journalJournal of Clinical & Laboratory Immunologyen
html.description.abstractCell-mediated haemolysis of human erythrocytes (HRBC) mediated by sensitizing alloantibodies of two specificities was studied using the 51Cr release assay. Peripheral blood mononuclear cells (PBMC) were found capable fo lysing HRBC of phenotype ARh(D) + ve sensitized with either anti-D or "natural" anti-A antibodies. The characteristics of the effector population, however, were dependent upon the sensitizing antibody. HRBC sensitized with anti-A were lysed by a radioresistant, silica- and carrageenan-sensitive population which could be selectively removed by adherence. Sensitization with rhesus antibody induced cytotoxicity by a population which was radiosensitive and relatively unaffected by removal of adherent cells or by treatment with silica or carrageenan. The results demonstrate that the antigen-antibody interaction, rather than the target cell type, determines which effector cells participate in antibody-dependent cellular cytotoxicity (ADCC) against human red cells.


This item appears in the following Collection(s)

Show simple item record