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dc.contributor.authorMcMillan, S
dc.contributor.authorFox, Margaret
dc.date.accessioned2011-06-22T09:04:46Z
dc.date.available2011-06-22T09:04:46Z
dc.date.issued1981-07
dc.identifier.citationEffects of metabolic inhibitors on cell lethality and mutation induction in Chinese hamster cells. I. Inhibitors of de novo purine synthesis and a comparison with the effects of caffeine. 1981, 36 (1):71-88 Chem Biol Interacten
dc.identifier.issn0009-2797
dc.identifier.pmid7249151
dc.identifier.doi10.1016/0009-2797(81)90030-2
dc.identifier.urihttp://hdl.handle.net/10541/134153
dc.description.abstractThe effect of pre- and posttreatment incubation of UV-irradiated and ethyl methanesulphonate (EMS) treated cells with non-toxic concentrations of inhibitors of de novo purine synthesis (dnPS) on expression of potentially lethal and premutational damage at the hypoxanthine-guanine phosphoribosyl transferase (HGPRT) locus in V79 cells has been examined. The concentrations of inhibitors used were shown to profoundly perturb de novo DNA synthesis, by measurements of [14C]formate uptake, and cell cycle progression by flow cytofluorimetry. Postincubation in 6-methyl mercapto-purine ribonucleoside (MMPR) usually but not invariably potentiated the cytotoxic effect of UV and EMS but azaserine (AZS) and methotrexate (MTX) were without effect. No effects on mutant frequencies were observed on posttreatment with any of these agents. Caffeine produced the least effect on dnPS, but invariably potentiated lethal damage. This potentiation of lethal damage is not mediated by dnPS inhibition as has been suggested for Chinese hamster ovary (CHO) cells.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshAzaserine
dc.subject.meshCaffeine
dc.subject.meshCell Line
dc.subject.meshCricetinae
dc.subject.meshCricetulus
dc.subject.meshEthyl Methanesulfonate
dc.subject.meshLung
dc.subject.meshMethotrexate
dc.subject.meshMethylthioinosine
dc.subject.meshMutation
dc.subject.meshPurines
dc.subject.meshUltraviolet Rays
dc.titleEffects of metabolic inhibitors on cell lethality and mutation induction in Chinese hamster cells. I. Inhibitors of de novo purine synthesis and a comparison with the effects of caffeine.en
dc.typeArticleen
dc.contributor.departmentPaterson Laboratories, Christie Hospital & Holt Radium Institute, Manchester, M20 9BX, (United Kingdom)en
dc.identifier.journalChemico-Biological Interactionsen
html.description.abstractThe effect of pre- and posttreatment incubation of UV-irradiated and ethyl methanesulphonate (EMS) treated cells with non-toxic concentrations of inhibitors of de novo purine synthesis (dnPS) on expression of potentially lethal and premutational damage at the hypoxanthine-guanine phosphoribosyl transferase (HGPRT) locus in V79 cells has been examined. The concentrations of inhibitors used were shown to profoundly perturb de novo DNA synthesis, by measurements of [14C]formate uptake, and cell cycle progression by flow cytofluorimetry. Postincubation in 6-methyl mercapto-purine ribonucleoside (MMPR) usually but not invariably potentiated the cytotoxic effect of UV and EMS but azaserine (AZS) and methotrexate (MTX) were without effect. No effects on mutant frequencies were observed on posttreatment with any of these agents. Caffeine produced the least effect on dnPS, but invariably potentiated lethal damage. This potentiation of lethal damage is not mediated by dnPS inhibition as has been suggested for Chinese hamster ovary (CHO) cells.


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