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    Effects of metabolic inhibitors on cell lethality and mutation induction in Chinese hamster cells. I. Inhibitors of de novo purine synthesis and a comparison with the effects of caffeine.

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    Authors
    McMillan, S
    Fox, Margaret
    Affiliation
    Paterson Laboratories, Christie Hospital & Holt Radium Institute, Manchester, M20 9BX, (United Kingdom)
    Issue Date
    1981-07
    
    Metadata
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    Abstract
    The effect of pre- and posttreatment incubation of UV-irradiated and ethyl methanesulphonate (EMS) treated cells with non-toxic concentrations of inhibitors of de novo purine synthesis (dnPS) on expression of potentially lethal and premutational damage at the hypoxanthine-guanine phosphoribosyl transferase (HGPRT) locus in V79 cells has been examined. The concentrations of inhibitors used were shown to profoundly perturb de novo DNA synthesis, by measurements of [14C]formate uptake, and cell cycle progression by flow cytofluorimetry. Postincubation in 6-methyl mercapto-purine ribonucleoside (MMPR) usually but not invariably potentiated the cytotoxic effect of UV and EMS but azaserine (AZS) and methotrexate (MTX) were without effect. No effects on mutant frequencies were observed on posttreatment with any of these agents. Caffeine produced the least effect on dnPS, but invariably potentiated lethal damage. This potentiation of lethal damage is not mediated by dnPS inhibition as has been suggested for Chinese hamster ovary (CHO) cells.
    Citation
    Effects of metabolic inhibitors on cell lethality and mutation induction in Chinese hamster cells. I. Inhibitors of de novo purine synthesis and a comparison with the effects of caffeine. 1981, 36 (1):71-88 Chem Biol Interact
    Journal
    Chemico-Biological Interactions
    URI
    http://hdl.handle.net/10541/134153
    DOI
    10.1016/0009-2797(81)90030-2
    PubMed ID
    7249151
    Type
    Article
    Language
    en
    ISSN
    0009-2797
    ae974a485f413a2113503eed53cd6c53
    10.1016/0009-2797(81)90030-2
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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