Investigation of the effects of the phorbol ester TPA on carcinogen-induced forward mutagenesis to 6-thioguanine-resistance in V79 Chinese hamster cells.
Authors
Kinsella, Anne RAffiliation
Paterson Laboratories, Christie Hospital and Holt Radium Institute, Withington, Manchester M20 9BX, UKIssue Date
1981
Metadata
Show full item recordAbstract
12-O-Tetradecanoyl-phorbol-13-acetate (TPA) was tested for its effect on N'-methyl-N'-nitro-N-nitrosoguanidine (MNNG) induced forward mutagenesis to 6-thioguanine resistance (6TGR) in V79 Chinese hamster cells. Using a replating assay, no effect of TPA on the recovery of 6TGR mutants was observed. Previous reports of TPA enhancement of carcinogen-induced forward mutagenesis using the in situ mutagenesis assay, can be explained in terms of the influence of metabolic co-operation on mutant recovery. TPA is known to eliminate metabolic co-operation, an artefact of the in situ mutagenesis assay system, thus contributing to the enhancement of mutant recovery in TPA treated cultures. This investigation aims to demonstrate that TPA has no influence on mutagenesis per se, positive or negative enhancement of forward mutagenesis being solely a reflection of the mutagenesis assay employed.Citation
Investigation of the effects of the phorbol ester TPA on carcinogen-induced forward mutagenesis to 6-thioguanine-resistance in V79 Chinese hamster cells. 1981, 2 (1):43-7 CarcinogenesisJournal
CarcinogenesisDOI
10.1093/carcin/2.1.43PubMed ID
7273287Type
ArticleLanguage
enISSN
0143-3334ae974a485f413a2113503eed53cd6c53
10.1093/carcin/2.1.43