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dc.contributor.authorNeal, J Valerie
dc.contributor.authorPotten, Christopher S
dc.date.accessioned2011-06-21T15:48:37Z
dc.date.available2011-06-21T15:48:37Z
dc.date.issued1981-01
dc.identifier.citationDescription and basic cell kinetics of the murine pericryptal fibroblast sheath. 1981, 22 (1):19-24 Guten
dc.identifier.issn0017-5749
dc.identifier.pmid7461472
dc.identifier.doi10.1136/gut.22.1.19
dc.identifier.urihttp://hdl.handle.net/10541/134090
dc.description.abstractThe size of the pericryptal fibroblast sheath (PCFS) population was determined by scoring serial sections. There are 38 and 124 PCFS cells per murine small intestinal and colonic crypt, respectively. The cells of the PCFS are slightly weighted towards the lower two-thirds of the crypt in their distribution. The ratio of epithelial cells to PCFS cells is approximately 6.5:1. The in vivo cell kinetics were analysed under control and stressed (fasted-refed) conditions. The control labelling index increases from 8.9% in the small intestine and 7.0% in the colon to peaks 49% and 113% respectively above these values 24 hours after 3HTdR administration. Labelling is observed at all crypt levels equally, and no evidence of vertical migration of labelled PCFS cells was found. Colonic epithelial and PCFS cells show a similar pattern of response to feeding after a fast of 72 hours with respect to time, but a different distribution of response in terms of crypt position.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshCell Count
dc.subject.meshColon
dc.subject.meshEpithelial Cells
dc.subject.meshFibroblasts
dc.subject.meshIntestine, Small
dc.subject.meshMale
dc.subject.meshMice
dc.subject.meshMice, Inbred DBA
dc.subject.meshMitotic Index
dc.subject.meshStarvation
dc.titleDescription and basic cell kinetics of the murine pericryptal fibroblast sheath.en
dc.typeArticleen
dc.contributor.departmentPaterson Laboratories, Christie Hospital, and Holt Radium Institute, Manchesteren
dc.identifier.journalGuten
html.description.abstractThe size of the pericryptal fibroblast sheath (PCFS) population was determined by scoring serial sections. There are 38 and 124 PCFS cells per murine small intestinal and colonic crypt, respectively. The cells of the PCFS are slightly weighted towards the lower two-thirds of the crypt in their distribution. The ratio of epithelial cells to PCFS cells is approximately 6.5:1. The in vivo cell kinetics were analysed under control and stressed (fasted-refed) conditions. The control labelling index increases from 8.9% in the small intestine and 7.0% in the colon to peaks 49% and 113% respectively above these values 24 hours after 3HTdR administration. Labelling is observed at all crypt levels equally, and no evidence of vertical migration of labelled PCFS cells was found. Colonic epithelial and PCFS cells show a similar pattern of response to feeding after a fast of 72 hours with respect to time, but a different distribution of response in terms of crypt position.


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