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dc.contributor.authorVose, Brent M
dc.contributor.authorMoore, Michael
dc.date.accessioned2011-06-21T17:10:38Z
dc.date.available2011-06-21T17:10:38Z
dc.date.issued1981-10
dc.identifier.citationCultured human T-cell lines kill autologous solid tumours. 1981, 3 (4):237-41 Immunol Letten
dc.identifier.issn0165-2478
dc.identifier.pmid6975752
dc.identifier.urihttp://hdl.handle.net/10541/134082
dc.description.abstractLymphocytes from peripheral blood, lymph node, spleen and tumour of 7 patients with various carcinomas (2 lung, 3 colon, 1 gastric and 1 parotid tumour) were cultured for 15 days in conditioned media containing T-cell growth factor (TCGF; Interleukin 2) after which their cytotoxic activity against autologous tumour (and in some instances, autologous normal) cells and allogeneic tumour targets was evaluated in a short-term 51Cr-release assay. Significant cytotoxicity against autologous tumour targets was detected in at least one effector preparation from all of the patients, under conditions where, in some cases, other autologous cells (normal lung, PHA-transformed lymphocytes) were resistant. This cytotoxicity also generally extended to allogeneic tumour targets, but lysis of K562, a cell line sensitive to natural killing, occurred in only 3 of 19 effector cell preparations. The data are consistent with a polyclonal expansion of cytotoxic T-cells of tumour-bearing patients which includes the amplification of a population recognitive of antigens expressed on autologous neoplastic cells.
dc.language.isoenen
dc.subject.meshCell Line
dc.subject.meshCell Transformation, Neoplastic
dc.subject.meshCells, Cultured
dc.subject.meshColonic Neoplasms
dc.subject.meshCulture Media
dc.subject.meshCytotoxicity, Immunologic
dc.subject.meshHumans
dc.subject.meshLung Neoplasms
dc.subject.meshLymph Nodes
dc.subject.meshParotid Neoplasms
dc.subject.meshStomach Neoplasms
dc.subject.meshT-Lymphocytes
dc.titleCultured human T-cell lines kill autologous solid tumours.en
dc.typeArticleen
dc.contributor.departmentDepartment of Immunology, Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BX (U.K.)en
dc.identifier.journalImmunology Lettersen
html.description.abstractLymphocytes from peripheral blood, lymph node, spleen and tumour of 7 patients with various carcinomas (2 lung, 3 colon, 1 gastric and 1 parotid tumour) were cultured for 15 days in conditioned media containing T-cell growth factor (TCGF; Interleukin 2) after which their cytotoxic activity against autologous tumour (and in some instances, autologous normal) cells and allogeneic tumour targets was evaluated in a short-term 51Cr-release assay. Significant cytotoxicity against autologous tumour targets was detected in at least one effector preparation from all of the patients, under conditions where, in some cases, other autologous cells (normal lung, PHA-transformed lymphocytes) were resistant. This cytotoxicity also generally extended to allogeneic tumour targets, but lysis of K562, a cell line sensitive to natural killing, occurred in only 3 of 19 effector cell preparations. The data are consistent with a polyclonal expansion of cytotoxic T-cells of tumour-bearing patients which includes the amplification of a population recognitive of antigens expressed on autologous neoplastic cells.


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