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dc.contributor.authorLanotte, Michael
dc.contributor.authorAllen, Terence D
dc.contributor.authorDexter, T Michael
dc.date.accessioned2011-06-21T17:06:09Z
dc.date.available2011-06-21T17:06:09Z
dc.date.issued1981-08
dc.identifier.citationHistochemical and ultrastructural characteristics of a cell line from human bone-marrow stroma. 1981, 50:281-97 J Cell Scien
dc.identifier.issn0021-9533
dc.identifier.pmid7320070
dc.identifier.urihttp://hdl.handle.net/10541/134080
dc.description.abstractMorphological, enzymic and antigenic data are presented regarding a human bone-marrow stromal cell line maintained for 10 months and subcultured weekly. The main characteristics are a fibroblastoid morphology, diffuse growth in collagen gels, no colony formation in soft gel media, contact inhibition of growth and conversion to adipocytes when treated with hydrocortisone. The cells are non-phagocytic and membrane Fc receptors (i.e. aggregated human immunoglobulin G receptors) are absent, but they show diffuse cytoplasmic non-specific esterase activity, a strong acid phosphatase reaction, and a negative immunofluorescence (direct and indirect) against factor VIII antigen. Other cell lines also have been isolated and maintained in culture and present similar characteristics. These cell lines are thought to be derived from the acid-phosphatase-positive marrow stroma directly associated with bone trabecular matrix and probably represent a component of the haemopoietic inductive microenvironment. As such, they may provide a useful tool for studies in vitro of cell interactions and regulatory processes in the control of human bone-marrow haemopoiesis.
dc.language.isoenen
dc.subject.meshAdipose Tissue
dc.subject.meshBone Marrow
dc.subject.meshCell Differentiation
dc.subject.meshCell Division
dc.subject.meshCell Line
dc.subject.meshCulture Media
dc.subject.meshHumans
dc.subject.meshHydrocortisone
dc.subject.meshMicroscopy, Electron
dc.subject.meshMicroscopy, Electron, Scanning
dc.subject.meshPhagocytosis
dc.titleHistochemical and ultrastructural characteristics of a cell line from human bone-marrow stroma.en
dc.typeArticleen
dc.contributor.departmentDepartment of Experimental Haematology and Department of Ultrastructure, Paterson Laboratories, Christie Hospital and Holt Radium Institute, Withington, Manchester M20 9BX, Englanden
dc.identifier.journalJournal of Cell Scienceen
html.description.abstractMorphological, enzymic and antigenic data are presented regarding a human bone-marrow stromal cell line maintained for 10 months and subcultured weekly. The main characteristics are a fibroblastoid morphology, diffuse growth in collagen gels, no colony formation in soft gel media, contact inhibition of growth and conversion to adipocytes when treated with hydrocortisone. The cells are non-phagocytic and membrane Fc receptors (i.e. aggregated human immunoglobulin G receptors) are absent, but they show diffuse cytoplasmic non-specific esterase activity, a strong acid phosphatase reaction, and a negative immunofluorescence (direct and indirect) against factor VIII antigen. Other cell lines also have been isolated and maintained in culture and present similar characteristics. These cell lines are thought to be derived from the acid-phosphatase-positive marrow stroma directly associated with bone trabecular matrix and probably represent a component of the haemopoietic inductive microenvironment. As such, they may provide a useful tool for studies in vitro of cell interactions and regulatory processes in the control of human bone-marrow haemopoiesis.


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