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    Histochemical and ultrastructural characteristics of a cell line from human bone-marrow stroma.

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    Authors
    Lanotte, Michael
    Allen, Terence D
    Dexter, T Michael
    Affiliation
    Department of Experimental Haematology and Department of Ultrastructure, Paterson Laboratories, Christie Hospital and Holt Radium Institute, Withington, Manchester M20 9BX, England
    Issue Date
    1981-08
    
    Metadata
    Show full item record
    Abstract
    Morphological, enzymic and antigenic data are presented regarding a human bone-marrow stromal cell line maintained for 10 months and subcultured weekly. The main characteristics are a fibroblastoid morphology, diffuse growth in collagen gels, no colony formation in soft gel media, contact inhibition of growth and conversion to adipocytes when treated with hydrocortisone. The cells are non-phagocytic and membrane Fc receptors (i.e. aggregated human immunoglobulin G receptors) are absent, but they show diffuse cytoplasmic non-specific esterase activity, a strong acid phosphatase reaction, and a negative immunofluorescence (direct and indirect) against factor VIII antigen. Other cell lines also have been isolated and maintained in culture and present similar characteristics. These cell lines are thought to be derived from the acid-phosphatase-positive marrow stroma directly associated with bone trabecular matrix and probably represent a component of the haemopoietic inductive microenvironment. As such, they may provide a useful tool for studies in vitro of cell interactions and regulatory processes in the control of human bone-marrow haemopoiesis.
    Citation
    Histochemical and ultrastructural characteristics of a cell line from human bone-marrow stroma. 1981, 50:281-97 J Cell Sci
    Journal
    Journal of Cell Science
    URI
    http://hdl.handle.net/10541/134080
    PubMed ID
    7320070
    Type
    Article
    Language
    en
    ISSN
    0021-9533
    Collections
    All Paterson Institute for Cancer Research

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