Show simple item record

dc.contributor.authorLord, Brian I
dc.date.accessioned2011-06-20T23:18:43Z
dc.date.available2011-06-20T23:18:43Z
dc.date.issued1981-07
dc.identifier.citationThe relationship of G0 to the cell cycle of haemopoietic spleen colony-forming cells. 1981, 14 (4):425-31 Cell Tissue Kineten
dc.identifier.issn0008-8730
dc.identifier.pmid7261026
dc.identifier.doi10.1111/j.1365-2184.1981.tb00549.x
dc.identifier.urihttp://hdl.handle.net/10541/133915
dc.description.abstractNormal haemopoietic stem cells, defined here as spleen colony-forming units (CFUs), are slowly proliferating and are generally considered to spend most of their time in the non-proliferative G0-state. A series of experiments using various combinations of the stem cell proliferation inhibitor (NBME-IV) and stimulator (RBME-III) together with vinblastine as a mitotic blocking agent was designed to determine the location of the G0-state relative to the cell cycle of the CFUs. From a knowledge of the effects of these agents, the expected results from three different G0-cell cycle models were charted and compared with the observed proliferative behaviour of the CFUs following these treatments. It was concluded that the out-of-style G0-state is located at the end of the G1-phase of the cycle, so that on receiving a stimulatory signal, the CFUs can rapidly enter the DNA-synthesis phase.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshBiological Products
dc.subject.meshHematopoietic Stem Cells
dc.subject.meshInterphase
dc.subject.meshLymphokines
dc.subject.meshMale
dc.subject.meshMice
dc.subject.meshVinblastine
dc.titleThe relationship of G0 to the cell cycle of haemopoietic spleen colony-forming cells.en
dc.typeArticleen
dc.contributor.departmentPaterson Laboratories, Christie Hospital and Holt Radium Institute, Withington, Manchesteren
dc.identifier.journalCell and Tissue Kineticsen
html.description.abstractNormal haemopoietic stem cells, defined here as spleen colony-forming units (CFUs), are slowly proliferating and are generally considered to spend most of their time in the non-proliferative G0-state. A series of experiments using various combinations of the stem cell proliferation inhibitor (NBME-IV) and stimulator (RBME-III) together with vinblastine as a mitotic blocking agent was designed to determine the location of the G0-state relative to the cell cycle of the CFUs. From a knowledge of the effects of these agents, the expected results from three different G0-cell cycle models were charted and compared with the observed proliferative behaviour of the CFUs following these treatments. It was concluded that the out-of-style G0-state is located at the end of the G1-phase of the cycle, so that on receiving a stimulatory signal, the CFUs can rapidly enter the DNA-synthesis phase.


This item appears in the following Collection(s)

Show simple item record