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    The effects of bifunctional alkylating agents on DNA synthesis in sensitive and resistant Yoshida cells.

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    Authors
    Carr, Francis J
    Fox, Brian W
    Affiliation
    Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester, M20 9BX, (United Kingdom)
    Issue Date
    1982-08
    
    Metadata
    Show full item record
    Abstract
    The effects of the alkylating agents methylene dimethanesulphonate (MDMS), sulphur mustard (SM) and methyl methanesulphonate (MMS) on DNA synthesis have been investigated in Yoshida sarcoma cell lines sensitive (YS) and resistant (YR) to these agents. Measurement of overall DNA synthesis by [3H]thymidine uptake into DNA indicated that both MDMS and SM, but not MMS, induced a greater depression of DNA synthesis in YS cells than in YR cells. Analysis of the sizes of newly synthesised strands of DNA by pulse labelling and alkaline sucrose gradient sedimentation revealed that MDMS inhibited the initation of new replicons and also reduced the size of new DNA strands. The relative extents of inhibition of replicon initation in MDMS-treated YS and YR cells were similar and reduced by caffeine while YR cells were comparatively less sensitive to the effect of MDMS on the sizes of DNA molecules synthesised. Similarly, YR cells apparently synthesised longer DNA strands following SM treatment compared to YS cells suggesting an association between the size of DNA molecules synthesized and the sensitivity of Yoshida cells to bifunctional alkylating agents.
    Citation
    The effects of bifunctional alkylating agents on DNA synthesis in sensitive and resistant Yoshida cells. 1982, 95 (2-3):441-56 Mutat. Res.
    Journal
    Mutation Research
    URI
    http://hdl.handle.net/10541/133771
    DOI
    10.1016/0027-5107(82)90277-9
    PubMed ID
    7121494
    Type
    Article
    Language
    en
    ISSN
    0027-5107
    ae974a485f413a2113503eed53cd6c53
    10.1016/0027-5107(82)90277-9
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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