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    Studies on ehrlich ascites tumour cells: DNA synthesis, and template activity of chromatin for DNA polymerase.

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    Authors
    Baghdjian, R B
    Itzhaki, S
    Ockey, Charles H
    Itzhaki, Ruth F
    Affiliation
    Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BX, UK
    Issue Date
    1982-12-10
    
    Metadata
    Show full item record
    Abstract
    Chromatin obtained from Ehrlich ascites cells on different days after cell inoculation has been assayed for its template activity with added DNA polymerase I. We have found that the template activity is 2 times higher in 7-8 day cell chromatin than in 4-day chromatin. Studies with added polylysine indicate that this increase reflects an increase in initiation sites rather than in accessibility to the enzyme. We have measured the growth fraction, mitotic index and rate of DNA chain growth in the intact cells. The results show that there is a large decrease in growth fraction with age of tumour, the number of cells dropping out of cycle approximately doubling over the period studied. The overall rate of chain growth decreases in the later stages of growth but in a small proportion of cells there is an increase in rate with fewer replicons involved in DNA synthesis. We suggest that in the ascites cells there is a decrease in level of repair and replicative enzymes with age of tumour; this would account both for the increase in initiation sites in the chromatin DNA, for the decrease in number of cells in cycle and for the overall decreased rate of chain growth.
    Citation
    Studies on ehrlich ascites tumour cells: DNA synthesis, and template activity of chromatin for DNA polymerase. 1982, 49 (3):169-75 Mol. Cell. Biochem.
    Journal
    Molecular and Cellular Biochemistry
    URI
    http://hdl.handle.net/10541/133542
    PubMed ID
    7162507
    Type
    Article
    Language
    en
    ISSN
    0300-8177
    Collections
    All Paterson Institute for Cancer Research

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