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dc.contributor.authorLanotte, Michael
dc.contributor.authorScott, David
dc.contributor.authorDexter, T Michael
dc.contributor.authorAllen, Terence D
dc.date.accessioned2011-06-16T21:25:59Z
dc.date.available2011-06-16T21:25:59Z
dc.date.issued1982-05
dc.identifier.citationClonal preadipocyte cell lines with different phenotypes derived from murine marrow stroma: factors influencing growth and adipogenesis in vitro. 1982, 111 (2):177-86 J. Cell. Physiol.en
dc.identifier.issn0021-9541
dc.identifier.pmid7045143
dc.identifier.doi10.1002/jcp.1041110209
dc.identifier.urihttp://hdl.handle.net/10541/133537
dc.description.abstractWe have isolated continuously growing cell lines derived from mouse bone marrow stroma. These cell lines were independently obtained, and though they showed morphologies ranging from the epithelioid to the fibroblastoid patterns, they all differentiated into adipocytes. Subclones obtained from two cell lines had a very high frequency (90-100%) of differentiation into adipocytes after two or three weeks of arrested growth. Though extensive accumulation of lipid often mechanically impaired mitosis, the cells committed to adipocytes did not suffer an irreversible loss of proliferative capacity. Adipogenesis was obtained in conditions similar to those required for fat cell formation in long-term bone marrow culture. The cell lines were found to be insensitive to insulin as a signal of adipocyte differentiation. The ultrastructural characteristics of the preadipocytes and fat cells are also similar to those of the fat cells developing in long-term bone marrow culture. As such, these cell lines should prove useful for analysing cell/cell interactions in haemopoiesis.
dc.language.isoenen
dc.subject.meshAdipose Tissue
dc.subject.meshAnimals
dc.subject.meshBlood
dc.subject.meshBone Marrow Cells
dc.subject.meshCell Differentiation
dc.subject.meshCell Division
dc.subject.meshCell Line
dc.subject.meshClone Cells
dc.subject.meshCulture Media
dc.subject.meshDexamethasone
dc.subject.meshHematopoietic Stem Cells
dc.subject.meshHydrocortisone
dc.subject.meshInsulin
dc.subject.meshMice
dc.titleClonal preadipocyte cell lines with different phenotypes derived from murine marrow stroma: factors influencing growth and adipogenesis in vitro.en
dc.typeArticleen
dc.contributor.departmentPaterson Laboratories, Christie Hospital and Holt Radium Institute, Withington, Manchester M20 9BX, Englanden
dc.identifier.journalJournal of Cellular Physiologyen
html.description.abstractWe have isolated continuously growing cell lines derived from mouse bone marrow stroma. These cell lines were independently obtained, and though they showed morphologies ranging from the epithelioid to the fibroblastoid patterns, they all differentiated into adipocytes. Subclones obtained from two cell lines had a very high frequency (90-100%) of differentiation into adipocytes after two or three weeks of arrested growth. Though extensive accumulation of lipid often mechanically impaired mitosis, the cells committed to adipocytes did not suffer an irreversible loss of proliferative capacity. Adipogenesis was obtained in conditions similar to those required for fat cell formation in long-term bone marrow culture. The cell lines were found to be insensitive to insulin as a signal of adipocyte differentiation. The ultrastructural characteristics of the preadipocytes and fat cells are also similar to those of the fat cells developing in long-term bone marrow culture. As such, these cell lines should prove useful for analysing cell/cell interactions in haemopoiesis.


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