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    Clonal preadipocyte cell lines with different phenotypes derived from murine marrow stroma: factors influencing growth and adipogenesis in vitro.

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    Authors
    Lanotte, Michael
    Scott, David
    Dexter, T Michael
    Allen, Terence D
    Affiliation
    Paterson Laboratories, Christie Hospital and Holt Radium Institute, Withington, Manchester M20 9BX, England
    Issue Date
    1982-05
    
    Metadata
    Show full item record
    Abstract
    We have isolated continuously growing cell lines derived from mouse bone marrow stroma. These cell lines were independently obtained, and though they showed morphologies ranging from the epithelioid to the fibroblastoid patterns, they all differentiated into adipocytes. Subclones obtained from two cell lines had a very high frequency (90-100%) of differentiation into adipocytes after two or three weeks of arrested growth. Though extensive accumulation of lipid often mechanically impaired mitosis, the cells committed to adipocytes did not suffer an irreversible loss of proliferative capacity. Adipogenesis was obtained in conditions similar to those required for fat cell formation in long-term bone marrow culture. The cell lines were found to be insensitive to insulin as a signal of adipocyte differentiation. The ultrastructural characteristics of the preadipocytes and fat cells are also similar to those of the fat cells developing in long-term bone marrow culture. As such, these cell lines should prove useful for analysing cell/cell interactions in haemopoiesis.
    Citation
    Clonal preadipocyte cell lines with different phenotypes derived from murine marrow stroma: factors influencing growth and adipogenesis in vitro. 1982, 111 (2):177-86 J. Cell. Physiol.
    Journal
    Journal of Cellular Physiology
    URI
    http://hdl.handle.net/10541/133537
    DOI
    10.1002/jcp.1041110209
    PubMed ID
    7045143
    Type
    Article
    Language
    en
    ISSN
    0021-9541
    ae974a485f413a2113503eed53cd6c53
    10.1002/jcp.1041110209
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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