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dc.contributor.authorLord, Brian I
dc.contributor.authorWright, Eric G
dc.date.accessioned2011-06-16T21:52:09Z
dc.date.available2011-06-16T21:52:09Z
dc.date.issued1982
dc.identifier.citationInteraction of inhibitor and stimulator in the regulation of CFU-S proliferation. 1982, 6 (4):541-51 Leuk. Res.en
dc.identifier.issn0145-2126
dc.identifier.pmid7144231
dc.identifier.doi10.1016/0145-2126(82)90011-X
dc.identifier.urihttp://hdl.handle.net/10541/133521
dc.description.abstractAn inhibitor and stimulator of CFU-S proliferation can be obtained from haemopoietic tissue containing, respectively, relatively quiescent CFU-S (e.g. normal bone marrow) and proliferating CFU-S (e.g. regenerating bone marrow). In this paper we explore the capacity for inhibitor and stimulator production in relation to changes in the proliferative status of the CFU-S and their mode of interaction. The increase in proliferative activity of CFU-S following a concentrated regime of phenylhydrazine injections is paralleled by an increase in the capacity for stimulator production and a decrease in the capacity for inhibitor production. These processes are reversed as the normal low proliferative activity returns. Using density fractionated marrow populations, dose-response measurements show that while inhibitor- and stimulator-producing cells persist throughout the changes in CFU-S proliferation, their magnitude of factor production moderates in relation to those changes. The inhibitor and stimulator are not mutually destructive: the two factors retain their activities after co-incubation and re-separation. On the other hand, the presence of either factor blocks the synthesis of the other by the appropriate producer cells. A model for the regulation of CFU-S proliferation by the inhibitor and stimulator is thus suggested in which the relative spatial distributions of CFU-S in a microenvironment containing inhibitor- and stimulator-producing cells are an important feature. It also implies the existence of a signal from the CFU-S compartment which determines the appropriate inhibitor or stimulator production.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshBone Marrow
dc.subject.meshCell Division
dc.subject.meshCell Fractionation
dc.subject.meshCulture Media
dc.subject.meshGrowth Inhibitors
dc.subject.meshGrowth Substances
dc.subject.meshHematopoietic Stem Cells
dc.subject.meshMale
dc.subject.meshMice
dc.subject.meshPhenylhydrazines
dc.subject.meshRegeneration
dc.titleInteraction of inhibitor and stimulator in the regulation of CFU-S proliferation.en
dc.typeArticleen
dc.contributor.departmentPaterson Laboratories, Christie Hospital and Holt Radium Institute, Withington, Manchester, M20 9BX, UKen
dc.identifier.journalLeukemia Researchen
html.description.abstractAn inhibitor and stimulator of CFU-S proliferation can be obtained from haemopoietic tissue containing, respectively, relatively quiescent CFU-S (e.g. normal bone marrow) and proliferating CFU-S (e.g. regenerating bone marrow). In this paper we explore the capacity for inhibitor and stimulator production in relation to changes in the proliferative status of the CFU-S and their mode of interaction. The increase in proliferative activity of CFU-S following a concentrated regime of phenylhydrazine injections is paralleled by an increase in the capacity for stimulator production and a decrease in the capacity for inhibitor production. These processes are reversed as the normal low proliferative activity returns. Using density fractionated marrow populations, dose-response measurements show that while inhibitor- and stimulator-producing cells persist throughout the changes in CFU-S proliferation, their magnitude of factor production moderates in relation to those changes. The inhibitor and stimulator are not mutually destructive: the two factors retain their activities after co-incubation and re-separation. On the other hand, the presence of either factor blocks the synthesis of the other by the appropriate producer cells. A model for the regulation of CFU-S proliferation by the inhibitor and stimulator is thus suggested in which the relative spatial distributions of CFU-S in a microenvironment containing inhibitor- and stimulator-producing cells are an important feature. It also implies the existence of a signal from the CFU-S compartment which determines the appropriate inhibitor or stimulator production.


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