Interaction of inhibitor and stimulator in the regulation of CFU-S proliferation.
AffiliationPaterson Laboratories, Christie Hospital and Holt Radium Institute, Withington, Manchester, M20 9BX, UK
MetadataShow full item record
AbstractAn inhibitor and stimulator of CFU-S proliferation can be obtained from haemopoietic tissue containing, respectively, relatively quiescent CFU-S (e.g. normal bone marrow) and proliferating CFU-S (e.g. regenerating bone marrow). In this paper we explore the capacity for inhibitor and stimulator production in relation to changes in the proliferative status of the CFU-S and their mode of interaction. The increase in proliferative activity of CFU-S following a concentrated regime of phenylhydrazine injections is paralleled by an increase in the capacity for stimulator production and a decrease in the capacity for inhibitor production. These processes are reversed as the normal low proliferative activity returns. Using density fractionated marrow populations, dose-response measurements show that while inhibitor- and stimulator-producing cells persist throughout the changes in CFU-S proliferation, their magnitude of factor production moderates in relation to those changes. The inhibitor and stimulator are not mutually destructive: the two factors retain their activities after co-incubation and re-separation. On the other hand, the presence of either factor blocks the synthesis of the other by the appropriate producer cells. A model for the regulation of CFU-S proliferation by the inhibitor and stimulator is thus suggested in which the relative spatial distributions of CFU-S in a microenvironment containing inhibitor- and stimulator-producing cells are an important feature. It also implies the existence of a signal from the CFU-S compartment which determines the appropriate inhibitor or stimulator production.
CitationInteraction of inhibitor and stimulator in the regulation of CFU-S proliferation. 1982, 6 (4):541-51 Leuk. Res.
- Stimulation of haemopoietic stem cell proliferation: characteristics of the stimulator-producing cells.
- Authors: Wright EG, Ali AM, Riches AC, Lord BI
- Issue date: 1982
- Effect of haemopoietic stem-cell proliferation regulators on early and late spleen colony-forming cells.
- Authors: Wright EG, Lorimore SA, Lord BI
- Issue date: 1985 Mar
- A stimulator of mouse stem cell proliferation produced by human regenerating bone marrow.
- Authors: Oishi H, Katsuno M, Umemura T, Nishimura J, Motomura S, Ibayashi H
- Issue date: 1987
- Production of stem cell proliferation stimulators and inhibitors by haemopoietic cell suspensions.
- Authors: Wright EG, Lord BI
- Issue date: 1978 May-Jun
- Mechanisms of haemopoietic stem cell proliferation control.
- Authors: Wright EG, Lord BI, Dexter TM, Lajtha LG
- Issue date: 1979 Jun 15