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dc.contributor.authorWynne, K M
dc.contributor.authorMoore, Michael
dc.date.accessioned2011-06-16T21:41:26Z
dc.date.available2011-06-16T21:41:26Z
dc.date.issued1982-09
dc.identifier.citationSmall-scale isolation and characterisation of human peripheral blood monocytes. 1982, 138 (1):1-16 J. Patholen
dc.identifier.issn0022-3417
dc.identifier.pmid7153814
dc.identifier.doi10.1002/path.1711380102
dc.identifier.urihttp://hdl.handle.net/10541/133519
dc.description.abstractA technique is described for the isolation and characterisation of monocytes from 15 ml of human peripheral blood. After density gradient centrifugation over Ficoll-Hypaque, monocytes are purified further by substrate adherence under carefully defined conditions. The use of a simple microwell slide system permits the production of multiple small-scale monolayer populations which can be characterised further in terms of their histochemical reactivity (combined stain for chloroacetate and non-specific esterases); endocytic capacity (latex particles); Fc (sensitised ox erythrocytes) and C3 (serum-coated yeast) receptor expression; and precursor maturation potential (7-day cultures). Evidence of considerable cellular heterogeneity is presented.
dc.language.isoenen
dc.subject.meshCell Separation
dc.subject.meshCells, Cultured
dc.subject.meshCentrifugation, Density Gradient
dc.subject.meshComplement C3
dc.subject.meshEndocytosis
dc.subject.meshHumans
dc.subject.meshMonocytes
dc.subject.meshReceptors, Complement
dc.subject.meshReceptors, Fc
dc.titleSmall-scale isolation and characterisation of human peripheral blood monocytes.en
dc.typeArticleen
dc.contributor.departmentImmunology Division, Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BXen
dc.identifier.journalJournal of Pathologyen
html.description.abstractA technique is described for the isolation and characterisation of monocytes from 15 ml of human peripheral blood. After density gradient centrifugation over Ficoll-Hypaque, monocytes are purified further by substrate adherence under carefully defined conditions. The use of a simple microwell slide system permits the production of multiple small-scale monolayer populations which can be characterised further in terms of their histochemical reactivity (combined stain for chloroacetate and non-specific esterases); endocytic capacity (latex particles); Fc (sensitised ox erythrocytes) and C3 (serum-coated yeast) receptor expression; and precursor maturation potential (7-day cultures). Evidence of considerable cellular heterogeneity is presented.


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