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    Small-scale isolation and characterisation of human peripheral blood monocytes.

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    Authors
    Wynne, K M
    Moore, Michael
    Affiliation
    Immunology Division, Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BX
    Issue Date
    1982-09
    
    Metadata
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    Abstract
    A technique is described for the isolation and characterisation of monocytes from 15 ml of human peripheral blood. After density gradient centrifugation over Ficoll-Hypaque, monocytes are purified further by substrate adherence under carefully defined conditions. The use of a simple microwell slide system permits the production of multiple small-scale monolayer populations which can be characterised further in terms of their histochemical reactivity (combined stain for chloroacetate and non-specific esterases); endocytic capacity (latex particles); Fc (sensitised ox erythrocytes) and C3 (serum-coated yeast) receptor expression; and precursor maturation potential (7-day cultures). Evidence of considerable cellular heterogeneity is presented.
    Citation
    Small-scale isolation and characterisation of human peripheral blood monocytes. 1982, 138 (1):1-16 J. Pathol
    Journal
    Journal of Pathology
    URI
    http://hdl.handle.net/10541/133519
    DOI
    10.1002/path.1711380102
    PubMed ID
    7153814
    Type
    Article
    Language
    en
    ISSN
    0022-3417
    ae974a485f413a2113503eed53cd6c53
    10.1002/path.1711380102
    Scopus Count
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    All Paterson Institute for Cancer Research

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