Small-scale isolation and characterisation of human peripheral blood monocytes.
AffiliationImmunology Division, Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BX
MetadataShow full item record
AbstractA technique is described for the isolation and characterisation of monocytes from 15 ml of human peripheral blood. After density gradient centrifugation over Ficoll-Hypaque, monocytes are purified further by substrate adherence under carefully defined conditions. The use of a simple microwell slide system permits the production of multiple small-scale monolayer populations which can be characterised further in terms of their histochemical reactivity (combined stain for chloroacetate and non-specific esterases); endocytic capacity (latex particles); Fc (sensitised ox erythrocytes) and C3 (serum-coated yeast) receptor expression; and precursor maturation potential (7-day cultures). Evidence of considerable cellular heterogeneity is presented.
CitationSmall-scale isolation and characterisation of human peripheral blood monocytes. 1982, 138 (1):1-16 J. Pathol
JournalJournal of Pathology
- The effect of complement in adherent immune complexes on Fc and C3 receptor expression in human monocytes.
- Authors: Arend WP, Massoni RJ
- Issue date: 1981 Dec
- Effect of soluble immune complexes of Fc and C3 receptor-dependent phagocytosis by human monocytes.
- Authors: Kávai M, Sándor M, Szegedi G, Füst G, Gergely J
- Issue date: 1981 Nov
- Characterization of canine monocytes in vitro: increased receptor activity for Fc, C3, and heterologous erythrocytes.
- Authors: Lucas DL, Bowles CA, Robinson DM
- Issue date: 1980 Feb
- Development of functional complement receptors during in vitro maturation of human monocytes into macrophages.
- Authors: Newman SL, Musson RA, Henson PM
- Issue date: 1980 Nov
- The isolation and purification of human peripheral blood monocytes in cell suspension.
- Authors: Treves AJ, Yagoda D, Haimovitz A, Ramu N, Rachmilewitz D, Fuks Z
- Issue date: 1980