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dc.contributor.authorAllen, Terence D
dc.contributor.authorDexter, T Michael
dc.date.accessioned2011-04-17T08:46:09Z
dc.date.available2011-04-17T08:46:09Z
dc.date.issued1982
dc.identifier.citationUltrastructural aspects of erythropoietic differentiation in long-term bone marrow culture. 1982, 21 (2):86-94 Differentiationen
dc.identifier.issn0301-4681
dc.identifier.pmid7084572
dc.identifier.doi10.1111/j.1432-0436.1982.tb01201.x
dc.identifier.urihttp://hdl.handle.net/10541/128231
dc.description.abstractLong-term liquid cultures of mouse bone marrow produce stem cell (CFU-S) and differentiated granulocytes for many months. Addition of AMS (anaemic mouse serum) to the cultures almost entirely eliminates the granulopoietic activity and stimulates erythropoiesis, with full erythroid maturation and the production of adult haemoglobin. Ultrastructural analysis of in situ fixed material reveals the cell shape and surface morphology of the erythroid maturation series, and the generation of erythroblastic islands in vitro. Each erythroblastic island consists of one or more synchronously maturing cohorts of erythroid cells undergoing four or five divisions between proerythroblast and normoblast. Each island is centered on a macrophage, which interacts with the developing erythroid population in several ways. Expelled nuclei are phagocytosed by te macrophage, which also has large areas of closely apposed membrane with the erythroid cells, gap junctions, and possible reciprocal vesicular activity. Changes in the adherent layer (stromal cells) also occur with the transition from granulopoiesis to erythropoiesis. There is a reduction in the endothelial cell cover, and mobilisation of lipid from the granulopoietic associated apidocytes.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshBone Marrow
dc.subject.meshCell Differentiation
dc.subject.meshCells, Cultured
dc.subject.meshCulture Media
dc.subject.meshErythropoiesis
dc.subject.meshKinetics
dc.subject.meshMice
dc.subject.meshMice, Inbred DBA
dc.subject.meshMicroscopy, Electron
dc.subject.meshMicroscopy, Electron, Scanning
dc.subject.meshTime Factors
dc.titleUltrastructural aspects of erythropoietic differentiation in long-term bone marrow culture.en
dc.typeArticleen
dc.contributor.departmentPaterson Laboratories, Christie Hospital and Holt Radium Institute, Withington, Manchester, M20 9BX, UKen
dc.identifier.journalDifferentiationen
html.description.abstractLong-term liquid cultures of mouse bone marrow produce stem cell (CFU-S) and differentiated granulocytes for many months. Addition of AMS (anaemic mouse serum) to the cultures almost entirely eliminates the granulopoietic activity and stimulates erythropoiesis, with full erythroid maturation and the production of adult haemoglobin. Ultrastructural analysis of in situ fixed material reveals the cell shape and surface morphology of the erythroid maturation series, and the generation of erythroblastic islands in vitro. Each erythroblastic island consists of one or more synchronously maturing cohorts of erythroid cells undergoing four or five divisions between proerythroblast and normoblast. Each island is centered on a macrophage, which interacts with the developing erythroid population in several ways. Expelled nuclei are phagocytosed by te macrophage, which also has large areas of closely apposed membrane with the erythroid cells, gap junctions, and possible reciprocal vesicular activity. Changes in the adherent layer (stromal cells) also occur with the transition from granulopoiesis to erythropoiesis. There is a reduction in the endothelial cell cover, and mobilisation of lipid from the granulopoietic associated apidocytes.


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